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003 EG-GiCUC
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008 191120s2019 ua dh f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.10.10.M.Sc.2019.Gh.R
100 0 _aGhada Sayed Abdelaziz
245 1 0 _aRecognition of two species of genus chlamydia derived from sheep, goats and cattle :
_bChlamydia abortus and Chlamydia pecorum /
_cGhada Sayed Abdelaziz ; Supervised Wagih Armanious Gad , Ahmed Samir Mohamed , Hadia Abdelrehim Ali
246 1 5 _aتعرف على نوعين من جنس الكلاميديا مستمدين من الاغنام والماعزوالابقار :
_bكلاميديا ابورتس وكلاميديا بيكورم
260 _aCairo :
_bGhada Sayed Abdelaziz ,
_c2019
300 _a148 P. :
_bcharts , facsimiles ;
_c25cm
502 _aThesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology
520 _aThe chlamydiae are a diverse group of obligate intracellular Gram-negative bacteria that are known to infect a wide variety of host species. The present study wasconducted to detect the incidence of Chlamydia abortus and Chlamydia pecorumamong cattle, sheep and goats. It was carried out on two groups of animals: the first one consists of apparently healthy cattle (n=35), sheep (n=8) and goats (n=23). The second one consists of diseased cattle (n=14), sheep (n=21) and goats (n=18) with different clinical findings such as: respiratory disorders, keratoconjunctivitis, vaginitis, abortion and diarrheaand the source of specimens were fecal (n=40), nasal (n=30), ocular (n=3) and vaginal samples (n=18). Moreover samples from internal organs (n=10) and sera (n=65) were also collected.Criteria of samples examination for detection of Chlamydia abortus and Chlamydia pecorum were as follow: CFT was used for determination of Chlamydia abortus and Chlamydia pecorum antibodies and antigen, Indirect Immunofluorescence test was used to confirm the presence of inclusion bodies of Chlamydia spp., Chlamydia abortus and Chlamydia pecorum were isolated by inoculation in 7-day old ECE via yolk sac route, TEM was used to confirm the presence of inclusion bodies of Chlamydia abortus and Chlamydia pecorum in infected yolk sac andThe collected internal organs from animals were stained by Giemsa stain for detection of inclusion bodies of Chlamydia abortus and Chlamydia pecorum. Results of CFT reveal that serum samples of cattle, sheep and goats were positive for Chlamydia spp. antibodies in a rate of 85%, fecal samples in cattle, sheep and goats showed positive results for Chlamydia spp. antigen in a rate of 77.5%, while vaginal swabs in sheep and goats showed positive results for Chlamydia spp. antigen in a rate of 70%, whereasnasal swabs in cattle and sheep showed positive results for Chlamydia spp. antigen in a rate of 77% and all ocular swabs in sheep showed positive results for Chlamydia spp. antigen
530 _aIssued also as CD
653 4 _aChlamydia abortus
653 4 _aChlamydia pecorum
653 4 _aTransmission electron microscope
700 0 _aAhmed Samir Mohamed ,
_eSupervisor
700 0 _aHadia Abdelrehim Ali ,
_eSupervisor
700 0 _aWagih Armanious Gad ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aShimaa
_eCataloger
942 _2ddc
_cTH
999 _c75339
_d75339