000			03425cam a2200325 a 4500
003			EG-GiCUC
008			191120s2019 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.12.21.M.Sc.2019.Ma.S
100	0		_aMarwa Ibrahim Hassan Ahmed Eissa
245	1	0	_aStudying the effect of diet containing genetically modified soybean meal on rats / _cMarwa Ibrahim Hassan Ahmed Eissa ; Supervised Mona Mostafa Mohammed , Medhat Elhalawany , Mohammed Elsherbiny
246	1	5	_aدراسة تأثير النظام الغذائى الذى يحتوى كسب فول الصويا المعدله وراثيا علي الفئران
260			_aCairo : _bMarwa Ibrahim Hassan Ahmed Eissa , _c2019
300			_a105 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology
520			_aWith increasing concerns from the public about genetically modified organisms (GMO), the efficient detection of GMO and their derived products is becoming a necessity. Because Roundup ready became the most cultivated genetically modified (GM) crop in the world. The aim of this study compose of two parts. Firstly, to monitor the presence of soybean in Egyptian market and to assess the efficiency of DNA-based (PCR) detection methods for GMO screening; In this study, 23 soybean samples were collected and analysed. The PCR assay employs primers specific for 35S promoter. The results indicated that out of the 23 samples treated, 19 samples gave positive results with the 35S promoter. Our results could also confirm the presence of CP4-EPSPS gene in the 35S positive samples. Glyphosate residues were found in detectable concentrations in GM soybean samples. Secondly to study the effect of a diet containing genetically modified soybean on both male and female Wistar albino rat. The body weights, food consumption, hematology, serum biochemistry, organ weight and histopathological examination were compared between rats fed GM Soybean and those fed non-GM Soybean after consumption of test diets for 18 weeks. The examined biochemical blood parameters showed that the level of triglycerides (TG) was statistically significantly higher of the female treated groups (T1 & T2) and lactate dehydrogenase (LDH) was significantly higher in treated groups compared to their respective controls. An elevated levels of Alkaline phosphatase (ALP), Aspartate aminotransferase (AST), Alanine aminotransferase (ALT) and creatinine were observed in some of the treated groups. Histopathological examination of selected vital organs (liver, kidney, and pancreas) and testes or ovaries and female mammary gland revealed histological alterations among different treated groups (T1 & T2) with their respective controls. This study shows the necessity of efficient detection of un-authorized GMO of imported crops and their derived products in Egypt. Also, to revise the safety standards of consumption of diets containing GM soybean
530			_aIssued also as CD
653		4	_aGenetically modified soybean
653		4	_aGMO
653		4	_aRUR
700	0		_aMedhat Elhalawany , _eSupervisor
700	0		_aMohammed Elsherbiny , _eSupervisor
700	0		_aMona Mostafa Mohammed , _eSupervisor
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c75345 _d75345