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003			EG-GiCUC
005			20250223032446.0
008			191123s2019 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.08.06.M.Sc.2019.No.S
100	0		_aNourhan Mohamed Abdelgelil Abousetta
245	1	2	_aA study on phenotypic and genotypic characterization of enterobacteriaceae, including campylobacter species and helicobacter pylori Recovered from water reservoirs in the Giza Area / _cNourhan Mohamed Abdelgelil Abousetta ; Supervised Mohammad Abdelhalim Ramadan , Omneya Mohamed Helmy , Walaa Ahmed Alshareef
246	1	5	_aدراسة على التوصيف الظاهرى والجينى للبكتيريا المعوية : شاملة بكتيريا الكامبيلوباكتر والبكتيريا الحلزونية المعزولة من خزانات المياه فى محيط الجيزة
260			_aCairo : _bNourhan Mohamed Abdelgelil Abousetta , _c2019
300			_a170 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology
520			_aBackground: Epidemiological studies have proved an association between drinking water sources and the incidence of H. pylori and Campylobacter spp. infection in developing countries. Aim: To investigate the role of water reservoirs as a potential source of H. pylori and Campylobacter spp. infection in Giza area, Egypt. Methods: A total of 125 water samples, from drinking water reservoirs, were collected from 13 districts of Giza governorate, Egypt. Physicochemical properties of the collected water samples including turbidity, color, odor, pH and chloride ion were determined. The filters obtained from the filtration of water samples were inoculated on a panel of different culture media including selective columbia blood agar (SCBA), modified columbia urea agar (MCUA), modified charcoal cefoperazone deoxycholate agar (mCCDA); the recovered isolated colonies were further identified by confirmatory biochemical reactions, Microscan WalkAway system or MALDI-TOF MS. Water samples were cultured in selective brain heart infusion broth (BHIB) supplemented with 20% horse serum, and selective Bolton broth; genomic DNA was extracted from the incubated cultures using phenol/chloroform/isoamyl alcohol extraction method. DNeasy® PowerWater® Kit for genomic DNA extraction from filtered water samples was used. PCR was used for the detection of H. pylori by virulence genes namely: glmM, ureA, cagA, vacA and Campylobacter spp. by cadF gene. All PCR amplified products were further sequenced
530			_aIssued also as CD
653		4	_aCampylobacter
653		4	_aGiza
653		4	_aH. pylori
700	0		_aMohammad Abdelhalim Ramadan , _eSupervisor
700	0		_aOmneya Mohamed Helmy , _eSupervisor
700	0		_aWalaa Ahmed Alshareef , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c75418 _d75418