000			03094cam a2200349 a 4500
003			EG-GiCUC
005			20250223032454.0
008			191207s2019 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.11.19.Ph.D.2019.Ab.E
100	0		_aAbeer Khaled Mokhtar Abuelazayem
245	1	0	_aEffect of diclofenac sodium on the expression levels of adhesion-related genes (asa1 and efaA) and the EfrAB efflux pump genes in enterococcal clinical isolates / _cAbeer Khaled Mokhtar Abuelazayem ; Supervised Heba Hamed Arnaout , Marwa Salah Mostafa , Maha Mahmoud Kotb
246	1		_aفى المكورات المعوية السريرية EfrAB وجينات مضخات efaA و asa1 تأثير ديكلوفيناك الصوديوم على مستويات التعبير عن الجينات المرتبطة بالالتصاق
260			_aCairo : _bAbeer Khaled Mokhtar Abuelazayem , _c2019
300			_a117 P. : _bcharts , facimiles ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology
520			_aAntimicrobial drug resistance has become an emerging threat and a matter of concern worldwide. Many steps are being taken to reduce this problem, like gradually limiting antibiotics administration against pathogenic bacteria and searching for non-antibiotic compounds that have antibacterial activity through different mechanisms. The aim of this work was to evaluate the antibacterial effect of diclofenac sodium on enterococcal growth, ciprofloxacin resistance and biofilm forming ability. The effect of exposure of enterococcal isolates to diclofenac sodium on their expression of efflux pump genes (efrA and efrB) and adherence-related genes (asa1 and efaA) was also investigated. Fifty Enterococcal isolates were retrieved from 36 urine and 14 pus specimens. Antimicrobial activity of diclofenac and ciprofloxacin was determined by agar dilution method.Their effect on biofilm formation was determined by tissue culture plate method. The expression of asa1, efaA, efrA and efrB genes was determined by RT-PCR before and after exposure to diclofenac. Ciprofloxacin resistance was reported in 54% of the enterococcal isolates. Diclofenac was found to have antibacterial activity, as it inhibited the growth of enterococcal isolates at MIC of 400-800æg/ml. Although diclofenac induced a statistically significant reduction in the expression of efflux pump genes (efrA and efrB) (P < 0.001), it failed to reduce either ciprofloxacin resistance rate or ciprofloxacin MICs. Biofilm formation was reported in 64% of the isolates
530			_aIssued also as CD
653		4	_aCiprofloxacin
653		4	_aDiclofenac
653		4	_aEnterococci
700	0		_aHeba Hamed Arnaout , _eSupervisor
700	0		_aMaha Mahmoud Kotb , _eSupervisor
700	0		_aMarwa Salah Mostafa , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c75686 _d75686