| 000 | 03071cam a2200337 a 4500 | ||
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| 003 | EG-GiCUC | ||
| 005 | 20250223032514.0 | ||
| 008 | 200114s2019 ua dh f m 000 0 eng d | ||
| 040 |
_aEG-GiCUC _beng _cEG-GiCUC |
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| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aM.Sc | ||
| 099 | _aCai01.08.06.M.Sc.2019.Ra.B | ||
| 100 | 0 | _aRasha Ibrahim Mohamed Elshamy | |
| 245 | 1 | 0 |
_aBacteriological investigation on listeriamonocytogenes in Egyptian food samples with special reference to its resistance patterns / _cRasha Ibrahim Mohamed Elshamy ; Supervised Ramy Karam Aziz , Mohamed Abdalla Abdelmoneam |
| 246 | 1 | 5 | _aدراسات بكتريولوجية على بكتيريا الليستريا مونوسيتوجين مع التركيز على طرز المقاومة |
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_aCairo : _bRasha Ibrahim Mohamed Elshamy , _c2019 |
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| 300 |
_a63 P. : _bcharts , facimiles ; _c25cm |
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| 502 | _aThesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology | ||
| 520 | _aListeria monocytogenes is among the most important foodborne pathogens. It may enter food processing environments through raw materials, handlers or equipment and may persist due to ineffective cleaning or sanitation. The bacterium can be isolated from both frozen vegetables and fresh food substances. This study aimed to estimate the prevalence of L. monocytogenes in spices and frozen vegetables and screen for some virulence factors and drug-resistance determinants of the isolated bacteria. First, conventional microbiological methods were used for the isolation and identification of bacteria. Next, the identity of isolated bacteria was confirmed by molecular techniques, and the virulence genes iap and hlyA were identified by real-time polymerase chain reaction (PCR). The hemolytic activity of the isolates was assessed by cultivation on sheep blood agar. Furthermore, the antimicrobial susceptibility of confirmed L. monocytogenes isolates was tested by the disk diffusion method against 10 antibiotics. Out of 331 vegetable samples, 47 isolates were confirmed to contain L. monocytogenes, whereas none of 40 spice samples tested positive. All isolates were positive for iap and hlyA genes.Susceptibility testing indicated that all isolates were sensitive to trimethoprim/sulfamethoxazole, but only 36% were sensitive to penicillin G, while 100% and 70% showed intermediate resistance to chloramphenicol and erythromycin, respectively. All tested isolates were resistant to amoxicillin, gentamicin and norfloxacin; on the other hand, 90, 86 and 84% of the tested strains were resistant to ciprofloxacin, ceftazidime/clavulanic acid and amikacin, respectively | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aAntibiotic resistance | |
| 653 | 4 | _aListeria monocytogenes | |
| 653 | 4 | _aVirulence genes | |
| 700 | 0 |
_aMohamed Abdalla Abdelmoneam , _eSupervisor |
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| 700 | 0 |
_aRamy Karam Aziz , _eSupervisor |
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| 856 | _uhttp://172.23.153.220/th.pdf | ||
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_aNazla _eRevisor |
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_aShimaa _eCataloger |
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_c76339 _d76339 |
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