000			02809cam a2200349 a 4500
003			EG-GiCUC
005			20250223032546.0
008			200824s2020 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.07.13.M.Sc.2020.Ay.I
100	0		_aAya Magdy Mahmod
245	1	0	_aIn vitro production of virus free banana plantlets from naturally infected plant / _cAya Magdy Mahmod ; Supervised Amina Hamed Gomaa , Samy Elkosary , Ahmed Shawky
246	1	5	_a انتاج نبيتات موز خاليه من الفيروس معمليا من نباتات مصابه طبيعيا
260			_aCairo : _bAya Magdy Mahmod , _c2020
300			_a109 P. : _bcharts , facaimiles ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Pomology
520			_aThe aim of this study was to evaluate the efficacy of chemotherapy,thermotherapy and cryotherapy treatments to eradicate the banana bunchy top virus (BBTV) from naturally infected banana cv. Grandnan shoot tip explants grown in vitro.Chemotherapy treatments using four different chemical substances (as antiviral), ribavirin was used at 20.0, 40.0 and 60.0 mg/L,jasmonic acid was used at 0.5, 1.0 and 2.0 mg/L,salicylic acid was added at 17.3, 34.5 and 69.0 mg/L,benzoic acid was used at 18.0, 36.0 and 72.0 mg/L. Also antiviral chemicals free-medium was investigated as control medium.Four different combinations of citric and ascorbic acids were investigated 50mg/L citric acid +100mg/ L ascorbic acid,100mg/L citric acid + 200mg/L ascorbic acid, 200mg/L citric acid + 400mg/L ascorbic acid and 400mg/L citric acid + 800mg/L ascorbic acid. In thermotherapy experiments the infected shoot tip explants incubated at 26±2{u00B0}C (control), at 35±2{u00B0}C, 40±2{u00B0}C or 45±2{u00B0}C for four weeks. Also the intermittent thermotherapy on infected shoot tip explants was investigated as the infected shoot tip explants incubated at 26±2{u00B0}C (control), at 35±2{u00B0}C for 16h darkness and transferred to incubated at 26±2{u00B0} for 8h light,40±2{u00B0}C for 16h darkness and transferred to incubated at 26±2{u00B0} for 8h light or 45±2{u00B0}Cfor 16h darkness and transferred to incubated at 26±2{u00B0} for 8h light for four weeks.In cryotherapy experiment infected shoot tip explants were cultured on preculture medium for 48h
530			_aIssued also as CD
653		4	_aAntiviral
653		4	_aBBTV
653		4	_aVirus-free
700	0		_aAhmed Shawky , _eSupervisor
700	0		_aAmina Hamed Gomaa , _eSupervisor
700	0		_aSamy Elkosary , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c77356 _d77356