000			02713cam a2200313 a 4500
003			EG-GiCUC
008			201001s2019 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.12.21.M.Sc.2019.Ra.A
100	0		_aRazan Mohammed Hassan Mohammed
245	1	0	_aAssessment of the antitumor efficacy of annona muricata (Graviola) in breast and prostate human cancer cell lines / _cRazan Mohammed Hassan Mohammed ; Supervised Magda Mohamed Noshy , Hanan Ramadan Hamad
246	1	5	_aتقييم الفاعليه المضادة للأورام لأنونا ميركاتا (غرافيولا) فى خطوط خلايا سرطان الثدى والبروستاتا البشرية
260			_aCairo : _bRazan Mohammed Hassan Mohammed , _c2019
300			_a77 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology
520			_aBackground Graviola (Annona muricata) is a small tropical evergreen fruit tree that is widely grown in many tropical zones and used as traditional medicine to treat cancer and tumors. Graviola has been reported to be effective against various human tumors in vitro cultures. Although the underlying molecular mechanisms of its antitumor efficacy are not fully understood, it has been suggested that the induction of apoptosis in cancer cells may account for Graviola antitumor efficacy. Aim The present study aimed to investigate the antitumor efficacy of Graviola leaves extract and to explore its effect on the expression levels of certain apoptotic genes (P53, Bax and Bcl-2) in human breast cancer cell line (MCF-7) and human prostate cancer cell line (PC3). Since the DNA damage is likely to be the primary cause of apoptosis, this study also aimed to evaluate the potential of Graviola leaves extract to induce DNA damage in both human cancer cells. Materials and methods The anti-proliferative effect of the methanol extract of Graviola leaves was evaluated by neutral red uptake assay. Gene expression was assayed by using quantitative real time polymerase chain reaction (qRT-PCR) while DNA damage was estimated by using alkaline comet assay. Results The results revealed the cytotoxic potential of Graviola leaves extract against the studied human breast and prostate cancer cell lines
530			_aIssued also as CD
653		4	_aAnnona muricata (Graviola)
653		4	_aBreast
653		4	_aProstate human cancer
700	0		_aHanan Ramadan Hamad , _eSupervisor
700	0		_aMagda Mohamed Noshy , _eSupervisor
905			_aAmira _eCataloger
905			_aNazla _eRevisor
942			_2ddc _cTH
999			_c78011 _d78011