000			03039cam a2200349 a 4500
003			EG-GiCUC
005			20250223032622.0
008			201101s2020 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.12.21.M.Sc.2020.Ay.P
100	0		_aAya Saber Ibrahim Imam
245	1	4	_aThe potential role of adipose tissue-derived mediators as promoters for breast cancer progression / _cAya Saber Ibrahim Imam ; Supervised Mona Mostafa Mohamed , Sherif Abdelaziz Ibrahim , Mohamed Elsayed Elshinawi
246	1	5	_aالدور المحتمل لمشتقات وسائط الانسجة الدهنية كمحفزات لتفاقم سرطان الثدى
260			_aCairo : _bAya Saber Ibrahim Imam, _c2020
300			_a139 P . : _bcharts , facsmilies ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology
520			_anflammatory breast cancer (IBC) represents a deadly aggressive subtype of BC with unique clinical and pathological characteristics. Although hundreds of studies identified different molecular and cellular factors involved in IBC progression, the role of adipocytes is still not elucidated yet. The adipose tissue (AT) expresses a variety of adipokines, which contribute in tumorigenesis. All types of cancer including BC contain a unique sub-population of cells with specific properties that are responsible for maintaining tumor growth and relapse termed cancer stem cells (CSCs). A total of 44 women (13 IBC and 31 non-IBC) diagnosed with BC were enrolled in this study. Herein, the main aim of the present study was to establish ex-vivo model of AT rather than 2D model. Besides, characterize the prominent AT secreted cytokines, chemokines and growth factors in obese IBC vis-à-vis obese non-IBC patients using human cytokine antibody array. Furthermore, our study validated whether the predominant cytokines level is also elevated at the transcript level after explant culture employing quantitative PCR (qPCR). Importantly, we studied the impact of AT secretome on the expression of epithelial-mesenchymal transition markers such as Vimentin and E-cadherin as well as cancer stem cell markers including, CD24 and CD44 in MDA-MB-231 non-IBC and SUM-149 IBC cell lines using qPCR. Data were statistically analyzed using unpaired Student{u2019}s t-test. Our cytokine array results revealed that an overall significantly higher level of a panel of 28 cytokines secreted by AT of obese IBC when compared to that of obese non-IBC patients IBC patients
530			_aIssued also as CD
653		4	_aAdipose Tissue
653		4	_aBreast Cancer
653		4	_aInterleukin- 6
700	0		_aMohamed Elsayed Elshinaw , _eSupervisor
700	0		_aMona Mostafa Mohamed , _eSupervisor
700	0		_aSherif Abdelaziz Ibrahim , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aAmira _eCataloger
905			_aNazla _eRevisor
942			_2ddc _cTH
999			_c78498 _d78498