000			03162cam a2200349 a 4500
003			EG-GiCUC
005			20250223032637.0
008			201129s2020 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.12.21.M.Sc.2020.Al.E
100	0		_aAlshaimaa Tarek Ismail Abdelfatah
245	1	0	_aEffect of post-surgical tumor microenvironment fluid on the breast cancer stem cell properties / _cAlshaimaa Tarek Ismail Abdelfatah ; Supervised Mona Mostafa Mohamed , Sherif Abdelaziz Ibrahim , Mohamed Elsayed Elshinawi
246	1	5	_aتأثير سائل البيئة الدقيقة من مكان استئصال الورم بعد الجراحة على خصائص الخلايا الجذعية لسرطان الثدى
260			_aCairo : _bAlshaimaa Tarek Ismail Abdelfatah , _c2020
300			_a104 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology
520			_aModified radical mastectomy (MRM) one of conventional treatment of breast cancer was suggested by many evidences that sometimes there is a relation between surgery and tumor recurrence. Most of breast local recurrences occur at the same quadrant of the primary cancer, suggesting that the tumor recurrence may promote from residual cancer cells and/ or quiescent stem cells lasting after surgery in tissues left behind after MRM. CSCs, a subpopulation of cancer cells, are characterized by self-renewal, unlimited and high proliferative potential and apoptosis resistance and the expression of specific cell surface markers. Breast cancer chemo and/or radio resistance, local recurrences and distant metastasis may originate from residual tumor cells and cancer stem cells (CSCs) left behind in the cavity after breast cancer surgery. We hypothesized that the residual malignant cells or quiescent CSCs may be activated by post-surgical tumor microenvironment fluid resulting at the surgical site through factors that are released in response to inflammation. In the present study post-surgical tumor microenvironment fluid were collected within 24 hrs of breast surgery in sterile tubes and filtered. The proteomic composition of post-surgical tumor microenvironment fluid was characterized using RayBio{u2122}human cytokine antibody Post-surgical tumor microenvironment fluid was used to stimulate MDA-MB-231 to test its effect on cell viability by MTT assay. Additionally, expression of CD44/CD24 stem-like surface markers was evaluated for MDA-MB-231 and SUM-149 breast cancer cell lines using Flow Cytometry. Moreover, EMT marker E-cadherin was assessed using immunofluorescence
530			_aIssued also as CD
653		4	_aBreast cancer
653		4	_aPost-surgical tumor microenvironment fluid
653		4	_aSurgery
700	0		_aMohamed Elsayed Elshinawi , _eSupervisor
700	0		_aMona Mostafa Mohamed , _eSupervisor
700	0		_aSherif Abdelaziz Ibrahim , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c79010 _d79010