000			03251cam a2200325 a 4500
003			EG-GiCUC
008			201130s2020 ua d f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.08.06.M.Sc.2020.Na.S
100	0		_aNazly Reda Elsayed Moaty
245	1	2	_aA study on the potential in vitro activity of natural products on biofilm formation by clinical and environmental isolates of pseudomonas spp. / _cNazly Reda Elsayed Moaty ; Supervised Mohammed Abdelhaleem Ramadan , Lina Jamil Abdelhafez , Reham Samir Mohammed
246	1	5	_aدراسة معملية على المواد الطبيعية على تثبيط تكوين الغشاء الحيوى بواسطة عزلات من السودموناس سريرية و بيئية
260			_aCairo : _bNazly Reda Elsayed Moaty , _c2020
300			_a121 P. : _bcharts ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology
520			_aMultidrug-resistant opportunistic pathogen Pseudomonas aeruginosa produces surface-associated communities called biofilm, which protect pathogens by forming a complex permeability barrier for antibiotics. Biofilm formation contributes to persistence and chronic infection caused by Pseudomonas aeruginosa. In this study, we investigated the potential activity of Camellia sinensis (green tea), Olea europaea (olive), Styrax benzoin, Ocimum basilicum, Humulus lupulus, Ruta graveolans, and propolis extracts on biofilm formation, pyocyanin production and twitching motility of P. aeruginosa isolates. Moreover, the effect of green tea and olive leaf extracts were investigated on the transcription of some biofilm related genes. A total of 204 isolates of Pseudomonas spp. collected from Egyptian hospitals. Susceptibility test of the recovered isolates was done using disc diffusion method. The capability of biofilm formation by isolates was evaluated by the Congo red and 96 well polystyrene Microtiter plate methods. The minimum inhibitory concentration (MIC) of these herbal extracts investigated using the broth microdilution method. Sub-MIC of herbal extracts mixed with strong biofilm former-MDR Pseudomonas isolates to determine the percentage of biofilm inhibition. The expression of lasI, lasR, rhlI and rhlR genes in isolated strains was detected using real-time rt-PCR. Susceptibility test revealing that about 49% of the isolates were multidrug-resistant.More than 90% of the isolates were biofilmforming, 26% were strong biofilm producers, 39% were moderate biofilm producers, 25% were weak biofilm producers and 10% couldn{u2019}t form any detectable biofilm. Sub-inhibitory concentrations of plant extracts mixed with strong biofilm former- MDR Pseudomonas isolates to determine the percentage of biofilm inhibition
530			_aIssued also as CD
653		4	_aBiofilm
653		4	_aNatural products
653		4	_aPseudomonas
700	0		_aLina Jamil Abdelhafez , _eSupervisor
700	0		_aMohammed Abdelhaleem Ramadan , _eSupervisor
700	0		_aReham Samir Mohammed , _eSupervisor
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c79036 _d79036