000			02955cam a2200313 a 4500
003			EG-GiCUC
008			201220s2020 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.11.19.M.Sc.2020.Al.E
100	0		_aAlaa Mohamed Ibrahim Sayed
245	1	0	_aEffects of lactobacillus acidophilus and lactobacillus helveticus on biofilm formation by ESBL-producing uropathogenic escherichia coli / _cAlaa Mohamed Ibrahim Sayed ; Supervised Magda Ibrahim Mohamed Ayoub , Alaa Mohamed Reda Awad
246	1	5	_aمدى تأثير بكتيريا حمض اللاكتيك الأسيدوفيلاس و الهيلفيتيكس على تكوين الغشاء الحيوى لدى بكتيريا الايشريشيا كولاى المسببة لعدوى الجهاز البولى و المنتجة لإنزيمات البيتا لاكتاميز المقاوم للمضادات الحيوية واسعة المدى
260			_aCairo : _bAlaa Mohamed Ibrahim Sayed , _c2020
300			_a81 P . : _bcharts , facsmilies ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology
520			_aUropathogenic E. coli (UPEC) are important clinical pathogens and are considered as one of the major causes of community-acquired and hospitalacquired urinary tract infection. Unfortunately, the treatment of UPEC infections is becoming more difficult because isolates are increasingly resistant to commonly used antimicrobial agents. The potential ability of UPEC to form biofilm might explain its outstanding antibiotic resistance, survival properties and increased virulence. The aim of the present study was to assess the effect of cell free supernatants (CFS) of Lactobacillus acidophilus La-5 and Lactobacillus helveticus B-734 on the ability of ESBL-producing UPEC isolates to form biofilm in vitro and to eradicate the already formed biofilm of UPEC isolates. A total of 50 isolates of ESBL-producing UPEC, were recovered from different private laboratories, then subcultured on MacConkey{u2019}s medium and identified by conventional microbiological methods. Antibiotic susceptibility testing was performed by Kirby-Bauer disc diffusion method and confirmed for ESBL production. The ability of UPEC to produce biofilm was determined using congo red agar method and tissue culture plate method. CFS of L. acidophilus La-5 and L. helveticusB-734 were prepared and used to assess its effect on the ability of ESBL-producing UPEC isolates to form biofilm in vitro and the eradication of the already formed biofilm
530			_aIssued also as CD
653		4	_acoli
653		4	_aESBL
653		4	_aUropahogenic E
700	0		_aAlaa Mohamed Reda Awad , _eSupervisor
700	0		_aMagda Ibrahim Mohamed Ayoub , _eSupervisor
905			_aAmira _eCataloger
905			_aNazla _eRevisor
942			_2ddc _cTH
999			_c79272 _d79272