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008 210202s2020 ua do f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.07.14.M.Sc.2020.Me.M
100 0 _aMwessongo Saumu Zuberi
245 1 0 _aMicropropagation of potato /
_c Mwessongo Saumu Zuberi ; Supervised Sayed Fathey Elsayed , Sahar Samih Taha , Omaima Darwish Saleh
246 1 5 _aالإكثار الدق{u٠٦أأ}ق لمحصول البطاطس
260 _aCairo :
_bMwessongo Saumu Zuberi ,
_c2020
300 _a138 P . :
_bcharts , photographs ;
_c25cm
502 _aThesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Vegetable
520 _aThe present research was conducted in tissue culture lab., veg. crops department, Fac. of Agric., Cairo Univ., Giza, Egypt. It aimed to reach the proper protocol for in vitro production of virus-free microtubers of potato cvs. Santana, Herms, and Spunta under light or dark conditions, through applications of silver thiosulfate, abscisic acid, humic acid, maltose and sucrose. Tip meristems were excised from potato tuber seeds. All cultivars were repropagated after 4 weeks for 2 subcultures. At the first experiment, explants were cultured on MS medium with 0, 0.4, 0.8, 1.2, 1.6 or 2 mM of STS under dark or light. STS at 0.4 and 0.8 mM recorded the tallest microplants. The highest number of leaflets and branches per plantlet were obtained with 0.4mM STS. The highest microtuberazation % was recorded with STS at 0.4 mM under dark conditions, and at 2 mM under light one. At the second experiment, explants were cultured on MS medium with 0, 0.5, 1.0 or 1.5 mM of ABA under dark or light. The highest value of plantlet height was recorded at 0 mM ABA. In dark condition, the highest microtuberazation % was recorded in cvs. Santana and Spunta with 0.5 and 0 mM ABA, respectively. The untreated microplantlets under dark recorded the highest number of microtubers/plantlet in cv. Herms At the third experiment, 0, 2,4,6,8 ml HA/l were applied at MS medium. Untreated microplantlets (0 ml HA/l) recorded the highest values of plant height, number of leaflets and branches/plantlet. The application with 2 ml/lHA obtained the highest microtuberazation % either under dark or light conditions. The application with 6 ml HA recorded the lowest number of microtuber/plantlet under dark
530 _aIssued also as CD
653 4 _aIn vitro tuberization
653 4 _aPotato
653 4 _aSilver thiosulphate
700 0 _aOmaima Darwish Saleh ,
_eSupervisor
700 0 _aSahar Samih Taha ,
_eSupervisor
700 0 _aSayed Fathey Elsayed ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aAmira
_eCataloger
905 _aNazla
_eRevisor
942 _2ddc
_cTH
999 _c79791
_d79791