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003 | EG-GiCUC | ||
005 | 20250223032701.0 | ||
008 | 210202s2020 ua do f m 000 0 eng d | ||
040 |
_aEG-GiCUC _beng _cEG-GiCUC |
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041 | 0 | _aeng | |
049 | _aDeposite | ||
097 | _aM.Sc | ||
099 | _aCai01.07.14.M.Sc.2020.Me.M | ||
100 | 0 | _aMwessongo Saumu Zuberi | |
245 | 1 | 0 |
_aMicropropagation of potato / _c Mwessongo Saumu Zuberi ; Supervised Sayed Fathey Elsayed , Sahar Samih Taha , Omaima Darwish Saleh |
246 | 1 | 5 | _aالإكثار الدق{u٠٦أأ}ق لمحصول البطاطس |
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_aCairo : _bMwessongo Saumu Zuberi , _c2020 |
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_a138 P . : _bcharts , photographs ; _c25cm |
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502 | _aThesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Vegetable | ||
520 | _aThe present research was conducted in tissue culture lab., veg. crops department, Fac. of Agric., Cairo Univ., Giza, Egypt. It aimed to reach the proper protocol for in vitro production of virus-free microtubers of potato cvs. Santana, Herms, and Spunta under light or dark conditions, through applications of silver thiosulfate, abscisic acid, humic acid, maltose and sucrose. Tip meristems were excised from potato tuber seeds. All cultivars were repropagated after 4 weeks for 2 subcultures. At the first experiment, explants were cultured on MS medium with 0, 0.4, 0.8, 1.2, 1.6 or 2 mM of STS under dark or light. STS at 0.4 and 0.8 mM recorded the tallest microplants. The highest number of leaflets and branches per plantlet were obtained with 0.4mM STS. The highest microtuberazation % was recorded with STS at 0.4 mM under dark conditions, and at 2 mM under light one. At the second experiment, explants were cultured on MS medium with 0, 0.5, 1.0 or 1.5 mM of ABA under dark or light. The highest value of plantlet height was recorded at 0 mM ABA. In dark condition, the highest microtuberazation % was recorded in cvs. Santana and Spunta with 0.5 and 0 mM ABA, respectively. The untreated microplantlets under dark recorded the highest number of microtubers/plantlet in cv. Herms At the third experiment, 0, 2,4,6,8 ml HA/l were applied at MS medium. Untreated microplantlets (0 ml HA/l) recorded the highest values of plant height, number of leaflets and branches/plantlet. The application with 2 ml/lHA obtained the highest microtuberazation % either under dark or light conditions. The application with 6 ml HA recorded the lowest number of microtuber/plantlet under dark | ||
530 | _aIssued also as CD | ||
653 | 4 | _aIn vitro tuberization | |
653 | 4 | _aPotato | |
653 | 4 | _aSilver thiosulphate | |
700 | 0 |
_aOmaima Darwish Saleh , _eSupervisor |
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700 | 0 |
_aSahar Samih Taha , _eSupervisor |
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700 | 0 |
_aSayed Fathey Elsayed , _eSupervisor |
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856 | _uhttp://172.23.153.220/th.pdf | ||
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_aAmira _eCataloger |
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_aNazla _eRevisor |
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_2ddc _cTH |
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_c79791 _d79791 |