000			03334cam a2200349 a 4500
003			EG-GiCUC
005			20250223032705.0
008			210215s2020 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.07.06.Ph.D.2020.He.B
100	0		_aHeba Hamed Baioumy Maghrawy
245	1	0	_aBiotechnological potential of bacterial metabolites immobilized on irradiated nanoclay particles in relation to crop production / _cHeba Hamed Baioumy Maghrawy ; Supervised Samir Elgizawy , Refae Ibrahim Refae , Hussein Abdelkareem
246	1	5	_aإمكانية التطبيق الحيوى للنواتج البكتيرية المحملة على جزيئات الطمى النانونية المشععة وعلاقة ذلك بإنتاجية المحاصيل
260			_aCairo : _bHeba Hamed Baioumy Maghrawy , _c2020
300			_a179 P. : _bcharts , facimiles ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Agricultural Microbiology
520			_aPotato is one of the major food crops belonging to family Solanaceae. Black scurf and stem canker, caused by Rhizoctonia solani are destructive diseases of potato causing serious damage in most potato-producing areas throughout the world. Chemical pesticides are widely used to fungal pathogens control. However, the application of chemical pesticides is harmful to both the environment and human health. Therefore, the use of biocontrol agents (BCAs) is considered to be potentially sustainable, cost-effective and eco-friendly ways to control R. solani. Accordingly, 84 isolates of PGPR were recovered and screened for their ability to produce antifungal compound(s), from rhizosphere of healthy potato plant collected from different areas. One strain was selected based on its ability to suppress the growth of R. solani. Phylogenetic analysis of this strain based on 16S rRNA gene sequences showed highest similarity (99%) with Bacillus sp. GRP (GenBank accession number: MK030136). The Bacillus sp. GRP culture filtrate contained protease, diffusible antibiotic, hydrogen cyanide and siderophore, it was capable of inhibiting growth of the pathogen up to 15 days compared to 7 and 10 days for other Bacillus isolates. It also produced indole acetic acid which promoted plant growth. Morphological and structural changes that took place as a result of Bacillus sp. and R. solani interaction were evaluated using light, SEM and TEM. The results showed that Bacillus sp. caused loss of structural integrity, abnormal coiling, shriveling and lysis of the R. solani hyphae, in addition to complete cytoplasm and internal organelles depletion. Among four media tested, the maximum growth and antifungal activities were found in tryptone soya broth medium under static condition at 30{u00B0}C, pH7, inoculum density of 1% and 72 h incubation
530			_aIssued also as CD
653		4	_aBacillus sp. GRP
653		4	_aOptimization
653		4	_aRhizoctonia solani
700	0		_aHussein Abdelkareem , _eSupervisor
700	0		_aRefae Ibrahim Refae , _eSupervisor
700	0		_aSamir Elgizawy , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c79902 _d79902