000			02880cam a2200325 a 4500
003			EG-GiCUC
008			210301s2020 ua a f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.10.19.Ph.D.2020.En.V
100	0		_aEngy Taha Bayomy Mohamed
245	1	0	_aViral problems of tilapia fish in Egypt / _cEngy Taha Bayomy Mohamed ; Supervised Mohamed Abdelaziz Ahmed , Mohamed Moustafa Mohamed , Ausama Abdelraouf Abdelmoneim
246	1	5	_aالمشاكل الفيروسية فى اسماك البلطى فى مصر
260			_aCairo : _bEngy Taha Bayomy Mohamed , _c2020
300			_a176 P. : _bill ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Veterinary Hygiene and Management
520			_aIncidencesof mortalities in cultured Nile tilapia (Oreochromis niloticus) have had a negative impact on the maintenance of many tilapia hatcheries and farms for the past few years. The socioeconomic structure of Egyptian aquaculture is severely affected due to fish loss, feed waste and loss of job opportunities.There is little information about pathogenic fish viruses in Egypt although members of twelve virus families have been identified worldwide in wild and farmed fish species causing drastic losses. Efforts were directed to investigate the possible viral causes of these massive infections. Therefore, the development of the synthetic positive control standard is crucial for the survey of viruses causingmortality in cultured tilapia as a rapid, affordable, and reliable molecular diagnostic assay. It was synthesized in silico allowed the amplification of fragments from nervous necrosis virus (NNV) RNA 2 and segment 9 of tilapia lake virus (TiLV) that reported to be threat to tilapia aquaculture. TilV RNA was not detected in any of the extracts tested using RT-PCR. NNV strains phylogenetically related to red-spotted grouper NNV were detected in three tilapia hatcheries in the period of investigation (2018-2019). Clinical signs of naturally infected fish were consistent with classical NNV infections (abnormal swimming behavior and skin darkening). Electron micrographs revealed virions in the retina and brain. NNV RNA was also detected in the brood stocks{u2019} brains and ovaries indicating the vertical transmission possibility. Experimental infection of NNV-free fries resulted in 100% morbidity and mortality
530			_aIssued also as CD
653		4	_aBetanodavirus
653		4	_aMass mortality
653		4	_aOreochromis niloticus
700	0		_aAusama Abdelraouf Abdelmoneim , _eSuipervisor
700	0		_aMohamed Abdelaziz Ahmed , _eSuipervisor
700	0		_aMohamed Moustafa Mohamed , _eSuipervisor
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c80082 _d80082