000 02755cam a2200313 a 4500
003 EG-GiCUC
008 210321s2020 ua d f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aPh.D
099 _aCai01.10.09.Ph.D.2020.Mo.S
100 0 _aMohamed Hamdy Mohamed Aly Ebid
245 1 0 _aSome studies on brucellosis in sheep and goats in Gulf region /
_cMohamed Hamdy Mohamed Aly Ebid ; Supervised Amal Abdel Aziz Elmolla , Fayez Awadalla Salib
246 1 5 _aبعض الدراسات عن مرض الاجهاض المعدى فى الاغنام والماعز فى منطقة الخليج
260 _aCairo :
_bMohamed Hamdy Mohamed Aly Ebid ,
_c2020
300 _a202 :
_bcharts , facsimiles ;
_c25cm
502 _aThesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Internal Medicine and Infectious Diseases
520 _aThis study was performed to determine the sero-prevalence of sheep and goats (small ruminants) brucellosis in some areas of Arabian Gulf, using by media-culture, serological methods (Rose Bengal Plate test (RBPT), indirect enzyme-linked immunosorbent assay (i-ELISA), complement fixation test (CFT) plus blood polymerase chain reaction (PCR) and PCR for tissue samples from positive reactors. From 140 (73 sheep and 67 goats) tissue samples were collected and examined using media culture, it was found that, out of the 140-tissue sample collected, 67/140 (47.8%) were confirmed positive, and biovar 1 is dominant in area 1 and 3, while biovar 2 is dominant in area 2, The serological examination using RBPT revealed that the Infection rate was total (855/20067 (4.26%)) animals were sero-positive, by using i-ELISA, and CFT reveledthat out of the 855 RBPT-positive reactors, positively confirmed in 717/20067 (3.57%), 595/20067 (2.97%) and respectively, were confirmed as positive.By PCR blood for confirmation cleared out of 855 positives by RBPT, 264/20067 (1.32%) animals, from confirmed positive reactors of animal 140 (73 sheep and 67 goats) tissue samples were collected and examined using PCR, collected from the positive reactor (RBPT and CFT), it was found that, out of the 140-tissue sample collected, by using PCR found that out of the 140-tissue sample collected, 128/140 (91.4%) were confirmed positive. PCR also can differentiate between vaccinated rev-1 strain and field strain B. melitensis (field infection strain)
530 _aIssued also as CD
653 4 _aBrucellosis
653 4 _aGoats
653 4 _aSheep
700 0 _aAmal Abdelaziz Elmolla ,
_eSupervisor
700 0 _aFayez Awadalla Salib ,
_eSupervisor
905 _aNazla
_eRevisor
905 _aShimaa
_eCataloger
942 _2ddc
_cTH
999 _c80312
_d80312