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003			EG-GiCUC
005			20250223032725.0
008			210407s2021 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.10.05.Ph.D.2021.Al.P
100	0		_aAlaa Mohamed Ali
245	1	0	_aPathological, immunohistochemical and molecular studies on the curative role of synthetic cannabinoid receptors-2 agonist (AM1241) in induced hepatic and pulmonary fibrosis in male rats / _cAlaa Mohamed Ali ; Supervised Sahar Samir Mahmoud Abdelrahman , Osama Samir Zaky Eltawil
246	1	5	_a فى تليف الكبد والرئة المستحدث فى ذكور الجرذان (AM1241) -2دراسات باثولوجية وامينوهيستوكيميايئية وجزيئية على الدورالعلاجى للمحفزات المصنعة لمستقبلات القنب
260			_aCairo : _bAlaa Mohamed Ali , _c2021
300			_a137 P . : _bcharts , facsmilies ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Clinical Pathology
520			_aThe activation of cannabinoid receptor 2 (CB2) plays a pleiotropic role in the innate immunity and is considered a crucial mediator in liver and lung disease. This study aimed to explore the role of activating CB2 using synthetic agonist (AM1241) on suppressing the progress of liver fibrosis induced by two models; bile duct ligation (BDL) and TAA administration as well as lung fibrosis induced by bleomycin (BLM) instillation. The study also attempted to clarify the mechanisms of the antifibrotic effects of synthetic CB2 agonists; AM1241 on the ongoing both hepatic and pulmonary fibrosis. AM1241 was administrated to rats of the aforementioned three models in two doses (3 and 6 mg/kg) for 14 days (in BDL experiment) and 21days (for the other experiments). Liver function and oxidative stress markers, hepatic TNF-Ü, IL-6 and IL-10, IL-1Ý, TGF-Ý1, RT-qPCR expression of Toll like receptor 4 (TLR4), TGF-Ý1, Ü-SMA and microRNA- 155 (miR-155) genes, western blot for Vimentin and E-cadherin proteins, immunohistochemical expression of CD31, CD34, Ü-SMA and NF-mB as well as histopathology of liver tissue were all assessed. In lung experiment, miRNA-21, Myd88 and TLR4 genes expression were detected by RT-q-PCR, while collagen I, Smad2 and Myd88 protein levels were analyzed by western blot. In addition, antioxidant activity (SOD and GSH), pro-fibrotic (TGF-Ý1) and pro-inflammatory (IL- 4 and IL-13) cytokines were determined in lung tissue homogenate using ELISA kits. Histological examination and immunohistochemical (IHC) evaluation of CD4, Ü-SMA and TGF- Ý1 expression were also examined. In both liver models; AM1241 administration at both doses significantly (P<0.05) ameliorated liver function markers, pro-inflammatory cytokines (TNF-Ü, IL-6), significantly decreased i GSH content and TLR4 gene expression
530			_aIssued also as CD
653		4	_aAM1241
653		4	_aCannabinoid receptor 2
653		4	_aTLR4
700	0		_aOsama Samir Zaky Eltawil , _eSupervisor
700	0		_aSahar Samir Mahmoud Abdelrahman , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aAmira _eCataloger
905			_aNazla _eRevisor
942			_2ddc _cTH
999			_c80554 _d80554