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| 005 | 20250223032729.0 | ||
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_aEG-GiCUC _beng _cEG-GiCUC |
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| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aPh.D | ||
| 099 | _aCai01.11.19.Ph.D.2021.Ay.M | ||
| 100 | 0 | _aAya Bakr Elesawi | |
| 245 | 1 | 0 |
_amazEF toxin-antitoxin system expression in methicillin resistant and methicillin sensitive staphylococcus aureus and its relation to biofilm-formation / _cAya Bakr Elesawi ; Supervised Heba Hamed Arnaout , Yasmine Samy Elkholy , Rania Yahia Shash |
| 246 | 1 | 5 | _aالسم ومضاد السم بين الكريات العنقودية الذهبية المقاومة و الحساسة للميثيسيلين وعلاقته بتكوين غشاء حيوى mazEF مقارنة نواتج النظام الجينى |
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_aCairo : _bAya Bakr Elesawi , _c2021 |
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_a105 P. : _bcharts , facsimiles ; _c25cm |
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| 502 | _aThesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology | ||
| 520 | _aBackground: Staphylococcus aureus is a common cause of many healthcare-associated and community-acquired infections. Methicillin resistance in S. aureus has become prevalent worldwide. Moreover biofilm-formation makes it more difficult to eradicate bacteria by antibiotics. mazEF toxin antitoxin system consists of a stable toxin called mazF which acts as an endoribonuclease and a labile antitoxin called mazE which opposes the toxin action. mazEF genes are located on S. aureus chromosome and can be used as an alternative target in treatment of infections. Objectives: The aim of the present work was to detect mazEF expression in MRSA isolates compared to MSSA isolates as well as determining mazEF system expression in relation to biofilm-formation. Methods: One hundred non-duplicate S. aureus isolates were collected from patients admitted to Kasr Al-Aini University Hospitals and categorized into 50 MRSA and 50 MSSA. All isolates were tested for antimicrobial susceptibility using disk diffusion method and biofilm-formation using tissue culture plate method. Finally, mazEF expression was detected by real time PCR and correlated to both methicillin susceptibility and biofilm-formation. Results: MRSA isolates were shown to be more resistant to antimicrobial agents tested than MSSA isolates. Linezolid and gentamycin showed the best sensitivity results with both MRSA and MSSA. 88% of MRSA and 96% of MSSA isolates were sensitive to linezolid while 76% of MRSA and 84% of MSSA isolates were sensitive to gentamycin. Biofilm-formation was statistically significantly higher in MRSA isolates than MSSA isolates (p value = 0.037). Expression of mazEF was statistically significantly higher in MRSA isolates compared to MSSA isolates (p value < 0.001), but no statistically significant correlation was detected between mazEF expression and biofilm-formation | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aMRSA | |
| 653 | 4 | _aMSSA | |
| 653 | 4 | _aToxin-antitoxin systems | |
| 700 | 0 |
_aHeba Hamed Arnaout , _eSupervisor |
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| 700 | 0 |
_aRania Yahia Shash , _eSupervisor |
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| 700 | 0 |
_aYasmine Samy Elkholy , _eSupervisor |
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| 856 | _uhttp://172.23.153.220/th.pdf | ||
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_aNazla _eRevisor |
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_aShimaa _eCataloger |
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_2ddc _cTH |
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_c80684 _d80684 |
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