| 000 | 03194cam a2200349 a 4500 | ||
|---|---|---|---|
| 003 | EG-GiCUC | ||
| 005 | 20250223032738.0 | ||
| 008 | 210520s2021 ua dh f m 000 0 eng d | ||
| 040 |
_aEG-GiCUC _beng _cEG-GiCUC |
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| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aPh.D | ||
| 099 | _aCai01.08.01.Ph.D.2021.Ma.S | ||
| 100 | 0 | _aMahmoud Taha Abdelaziz Aboelfadl | |
| 245 | 1 | 0 |
_aSynthetic lethality of triple negative breast cancer through trgeted silencing by small interfering RNA / _cMahmoud Taha Abdelaziz Aboelfadl ; Supervised Manal Fouad Ismail , Nancy Nabil Shahin , Amira M. Gamal Aldeen |
| 246 | 1 | 5 | _aالموت الاصطناعى لسرطان الثدى ثلاتى السلبية من خلال الاسكات الموجه باستخدام الحمض النووى الريبوزى المتداخل الصغير |
| 260 |
_aCairo : _bMahmoud Taha Abdelaziz Aboelfadl , _c2021 |
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| 300 |
_a262 P . : _bcharts facsmilies ; _c25cm |
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| 502 | _aThesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Biochemistry | ||
| 520 | _aBackground: TNFRSF13B, TACI, is a member of the TNF receptor superfamily; it plays a key role in cancer cell proliferation and progression. Method: Influence of silencing of human cytokine receptors on cell viability was screened by Luminescent Cell Viability Assay, after transfection with the siRNA library to find the maximum cell death superhits in both triple- negative MDAMB- 231 and triple-positive MCF-7 breast cells. The mode of cell death was investigated by dual DNA fluorescence staining. The expression of mRNAs of TACI, BAFF, BAFF-R, and APRIL was explored by qPCR. Immunocytofluorescence analysis was used to evaluate changes in TACI, Bcl-2, TNFR2, cyclin-D2, and PCNA. NF-kB p65, cell cycle, and necrosis/apoptosis (late and early) were analyzed by flowcytometry. Results: TACI was the most potent cytotoxic superhit resulting from highthroughput screening of the siRNA library, in both types of cells. Our findings indicated that silencing TACI receptor in both types of breast cancer cells led to significant cell death, after different intervals from siRNA transfection. Cell death mediators (TNF-R2, Bcl-2, and NF-mB p65) were significantly decreased after TACI silencing. The key factors for cell division (cyclin-D2 and PCNA) were significantly increased in silenced cells of both types but the cell cycle was arrested before the completion of mitosis. Expression of BAFF, BAFF-R and APRIL mRNA in TACI-silenced cells showed significant upregulation in MDAMB- 231 cells, while only BAFF-R and APRIL showed significant downregulation in MCF-7 cellsConclusion: TACI silencing can be a new and promising therapeutic strategy for mesenchymal-stem like triple- negative breast cancer subtype | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aRNA | |
| 653 | 4 | _aSynthetic lethality | |
| 653 | 4 | _aTriple negative breast cancer | |
| 700 | 0 |
_aAmira M. Gamal Aldeen, _eSupervising |
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| 700 | 0 |
_aManal Fouad Ismail , _eSupervising |
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| 700 | 0 |
_aNancy Nabil Shahin , _eSupervising |
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| 856 | _uhttp://172.23.153.220/th.pdf | ||
| 905 |
_aAmira _eCataloger |
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| 905 |
_aNazla _eRevisor |
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| 942 |
_2ddc _cTH |
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| 999 |
_c80960 _d80960 |
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