000			02954cam a2200349 a 4500
003			EG-GiCUC
005			20250223032744.0
008			210603s2021 ua d f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.10.15.Ph.D.2021.Ah.A
100	0		_aAhmed Nagy Mohamed
245	1	0	_aAdvanced Pharmacological Studies on the Progesterone Hormone in Mares and Cows / _cAhmed Nagy Mohamed ; Supervised Attia H. Atta , Abdelmohsen M. Hammam , Walter H. Hsu
246	1	5	_aدراسات فارماكولوجية متقدمة على هرمون البروجستيرون فى الافراس و الابقار
260			_aCairo : _bAhmed Nagy Mohamed , _c2021
300			_a150 P . : _bcharts ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Vet. Pharmacology
520			_aThe present study aimed to investigate the mRNA and protein expressions of nuclear progesterone (P4) receptors; PRA and PRB in mares and cows during the estrous cycle, pregnancy and parturition. RT-qPCR was used to quantify the mRNA expression for PRA and PRB and the difference between mRNA expressions for both genes was compared using the xxCt method. While the Western blot technique was used to compare the protein expression, the band intensity was analyzed using Image J. The tissue P4 concentration was measured using the enzyme immunoassay (EIA). The mRNA and protein expressions for PRA were significantly higher (P<0.0001) in equine placental tissue compared to the PRB mRNA and protein expressions. The expression of PRA mRNA in the cyclic mares was higher (P<0.005) during estrus than diestrus, however, there was no difference in the expression of PRB in the two phases. On the other hand, the protein expression for both PRA and PRB was higher (P<0.005) in estrus compared to the diestrus. The endometrial P4 concentration during diestrus was lower (P<0.005) than in estrus. Both PRA and PRB mRNA (P<0.005) and protein expression (P<0.0005) in cows' myometrial tissues decreased at term compared to the pregnancy. Also, the concentration of P4 in myometrial tissue decreased (P<0.005) in cows at parturition. The expression of mRNA for both isoforms PRA and PRB was high in the early luteal phase (ELP) (P<0.05) compared to the middle and late luteal phases (MLP and LLP). The protein expression of PRB was higher (P<0.05) in ELP compared to MLP and LLP. On the other hand, the protein expression of PRA did not change between all the phases
530			_aIssued also as CD
653		4	_aCows
653		4	_aProgesterone
653		4	_aReceptors
700	0		_aAbdelmohsen M. Hammam , _eSupervising
700	0		_aAttia H. Atta , _eSupervising
700	0		_aWalter H. Hsu , _eSupervising
856			_uhttp://172.23.153.220/th.pdf
905			_aAmira _eCataloger
905			_aNazla _eRevisor
942			_2ddc _cTH
999			_c81151 _d81151