| 000 | 03159cam a2200349 a 4500 | ||
|---|---|---|---|
| 003 | EG-GiCUC | ||
| 005 | 20250223032748.0 | ||
| 008 | 210612s2020 ua dh f m 000 0 eng d | ||
| 040 |
_aEG-GiCUC _beng _cEG-GiCUC |
||
| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aPh.D | ||
| 099 | _aCai01.09.14.Ph.D.2020.He.E | ||
| 100 | 0 | _aHebatollah Khaled Rizk | |
| 245 | 1 | 4 |
_aThe expression of the apoptosis-regulating mirnas 16 and 21 in oral squamous cell carcinoma : _bIn vitro study / _cHebatollah Khaled Rizk ; Supervised Naglaa Mohamed Elhosary , Safa Fathy Abdelghani , Olfat Shaker |
| 246 | 1 | 5 |
_aفى سرطان الخلايا الحرشفيه بالفم (miRNA 16,21 )التعبير الجينى لمنظمى موت الخلايا المبرمج : _bدراسه معمليه |
| 260 |
_aCairo : _bHebatollah Khaled Rizk , _c2020 |
||
| 300 |
_a115 P. : _bcharts , facsimiles ; _c25cm |
||
| 502 | _aThesis (Ph.D.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Oral and Maxillofacial Pathology | ||
| 520 | _aAim: to examine whether the expression of miRNA-16 and 21 (which are considered as apoptosis regulating miRNAs) alters in oral squamous cell carcinoma (OSCC) versus normal oral epithelium, and correlate their expression levels with that of one of their common downstream targets (the antiapoptotic BCL-2 protein). Methodology: this study was conducted on a total of 32 OSCC cases and 16 normal epithelium control cases. OSCC cases were graded as well, moderately, or poorly differentiated SCC, based on the degree of differentiation, cellular pleomorphism, and mitotic activity. Both moderately and poorly differentiated cases were placed as one group, whereas well differentiated cases were placed as a separate group. Quantitative real time polymerase chain reaction (qRT-PCR) was used to evaluate the expression of miRNA-16 and miRNA-21 in the 3 studied groups. The expression of BCL2 protein was evaluated by Immunohistochemical staining. Results: there was a statistically significant difference in miRNA-16 expression among the 3 studied groups, where it decreased gradually from the normal control group (mean 0.93) , followed by the well differentiated OSCC group (mean 0.36) , reaching the least levels in the moderate/poorly differentiated group (mean 0.17) . Also, the 3 groups showed a statistically significant difference in miRNA-21 expression, where the highest levels were observed in the moderate/poorly differentiated OSCC group (mean 9.35), followed by the well differentiated group (mean 3.70), and finally the normal control group (mean 0.96). However, all the studied groups showed negative BCL-2 immunostaining | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aOral squamous cell carcinoma (OSCC) | |
| 653 | 4 | _aPolymerase chain reaction (qRT-PCR) | |
| 653 | 4 | _aThe apoptosis-regulating mirnas 16 and 21 | |
| 700 | 0 |
_aNaglaa Mohamed Elhosary , _eSupervisor |
|
| 700 | 0 |
_aOlfat Shaker , _eSupervisor |
|
| 700 | 0 |
_aSafa Fathy Abdelghani , _eSupervisor |
|
| 856 | _uhttp://172.23.153.220/th.pdf | ||
| 905 |
_aNazla _eRevisor |
||
| 905 |
_aShimaa _eCataloger |
||
| 942 |
_2ddc _cTH |
||
| 999 |
_c81258 _d81258 |
||