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003			EG-GiCUC
005			20250223032750.0
008			210616s2021 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.11.19.Ph.D.2021.Ha.E
100	0		_aHagar Lotfy Abdelbaky Ahmad Mowafy
245	1	0	_aExpression profiles of circular PTPN22, microRNA-200a-3p and interleukin-2 in systemic lupus erythematosus shock / _cHagar Lotfy Abdelbaky Ahmad Mowafy ; Supervised Kamal Maurice Hanna , Lamiaa Abdelfattah Madkour, Maha Mahmoud Kotb
246	1	5	_aوالانترلوكين فى الذئبة الحمراء a200-3pوالحمض النووى الريبوزى المصغر circPTPN22قياس التعبير الجينى للحمض النووى الريبوزى الدائرى
260			_aCairo : _bHagar Lotfy Abdelbaky Ahmad Mowafy , _c2021
300			_a121 P . : _bcharts , facsimiles ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Microbiology and immunology
520			_aSystemic lupus erythematosus (SLE) is a typical autoimmune disease characterized by production of a variety of autoantibodies and various organs damage. Circular RNAs (circRNAs) are novel class of endogenous noncoding RNAs (ncRNAs) which have been involved in the development of various diseases. However, there is little knowledge about their associations with SLE. The aim of our study was to compare the plasma expression levels of circPTPN22, miR-200a-3p and IL-2 in SLE patients with that of healthy controls. The study was conducted on 45 SLE patients and 45 healthy individuals who served as the control group. The expression levels of circPTPN22 and miR-200a-3p were evaluated in the plasma of both groups by real time reverse transcription -polymerase chain reaction (RT-PCR). while plasma levels of IL-2 were determined using the enzyme-linked immunosorbent assay (ELISA) technique. SLE patients showed significantly lower plasma expression levels of circPTPN22 (P < 0.001), higher plasma expression levels of miR-200a-3p (P < 0.001) and lower plasma IL-2 levels (P < 0.001) compared to the control group. There was a statistically significant negative correlation between the expression levels of circPTPN22 and miR-200a-3p in the plasma of SLE patients (r = -0.567, P = < 0.001). However, no correlation was detected between the expression levels IL-2 and each of circPTPN22 (r = 0.170, P = 0.265) and miR-200a-3p (r = -0.284, P = 0.059). CircPTPN22, miR-200a-3p and IL-2 are potential non-invasive biomarkers in SLE diagnosis and potential therapeutic targets. Further studies are required to investigate the role of circPTPN22 and miR-200a-3p in the pathogenesis of SLE
530			_aIssued also as CD
653		4	_acircPTPN22
653		4	_aCircRNAs
653		4	_aIL-2
700	0		_aKamal Maurice Hanna , _eSupervising
700	0		_aLamiaa Abdelfattah Madkour, _eSupervising
700	0		_aMaha Mahmoud Kotb , _eSupervising
856			_uhttp://172.23.153.220/th.pdf
905			_aAmira _eCataloger
905			_aNazla _eRevisor
942			_2ddc _cTH
999			_c81314 _d81314