000			03465cam a2200325 a 4500
003			EG-GiCUC
008			210814s2021 ua d f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.07.04.Ph.D.2021.Mo.B
100	0		_aMosab Abdalla Ahmed Gad
245	1	0	_aBiochemical studies on some plant extracts as anti-cancer / _cMosab Abdalla Ahmed Gad ; Supervised Fouad Abdelrehim Ahmed , Mohamed Saad Abdellatiif , Hassan Zaki Hassouna
246	1	5	_aدراسات كيميائية حيوية على مستخلصات بعض النباتات كمضاد للسرطان
260			_aCairo : _bMosab Abdalla Ahmed Gad , _c2021
300			_a165 P. : _bcharts ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Agricultural Biochemistry
520			_aThe aim of this study was to evaluate the bioactivity of the extracts of the branch of Pinus roxburghii (P. roxburghii) and Nauplius graveolens (N. graveolens) as potential pharmaceutical anticancer drug against human colorectal cancer (HCT-116). The extracts of P. roxburghii and N. graveolens were tested for their antioxidant, antibacterial and antifungal activities. The N. graveolens possessed high significant scavenging activity against DPPH and ABTS assays relative to the moderate antioxidant activity exhibited by P. roxburghii. HPLC separation demonstrated that catechin and chlorogenic acid were predominant polyphenolic compounds in P. roxburghii and N. graveolens, respectively. Moreover, the mentioned extracts exhibited antimicrobial activity against all tested bacteria and fungi. The extracts inhibited the viability of HCT-116 cells with IC₅₀ values of 30.6 og ml-1and 26.5 og m⁻¹, respectively. DNA fragmentation was carried out by gel electrophoresis to detect the apoptotic pathway initiation and the results suggested that the P. roxburghii and N. graveolens extracts induced apoptosis in HCT-116 cells.The apoptotic pathway was tracked through measuring the relative level of mRNA expression of Bcl-2, Bax, Cas3, NF-mB, and PI3k genes by qRT-PCR technique. Using of the IC₅₀ doses of the selected extracts significantly up-regulated Bax, cleaved Cas-3 and down-regulated Bcl-2, NF-{u0138}B and PI3k genes versus GAPDH gene as a house keeping gene compared to control group. The Bax/Bcl-2 ratio was increased up on treatment. The potential toxicity of extracts of P. roxburghii and N. graveolens was assessed.The toxicity profile and the antitumor effect of the extracts of the two plants were tested in vivo in mice model. The results showed that the LD₅₀ of P. roxburghii was 1708.3 mg/ kg body weight and of N. graveolens 1562.5 mg/kg body weight.The results showed that this treatment led to a failure in the liver and kidney function tests. A second trial was designed, in which the mice were administered intragastrically day after day for a whole month with 1/10 the dose of the LD₅₀ of either plant extract
530			_aIssued also as CD
653		4	_aAntibacterial and Antifungal activities
653		4	_aColorectal cancer
653		4	_aP. roxburghii and N. graveolens extracts
700	0		_aFouad Abdelrehim Ahmed , _eSupervisor
700	0		_aHassan Zaki Hassouna , _eSupervisor
700	0		_aMohamed Saad Abdellatiif , _eSupervisor
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c81684 _d81684