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003			EG-GiCUC
005			20250223032815.0
008			210914s2021 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.10.10.Ph.D.2021.Am.M
100	0		_aAmal Mahmoud Abdu Mohamed
245	1	0	_aMolecular characterization of virulence genes of chlamydophila psittaci isolated from avian sources and its importance / _cAmal Mahmoud Abdu Mohamed ; Supervised Kamelia Mahmoud Osman , Hadia Abdelreheem Ali
246	1	5	_aالتوصيف الجزيئى لجينات الضراوة المصاحبة لعترات الكلاميدوفيلا سيتاسى المعزولة من الدواجن ومدى أهميتها
260			_aCairo : _bAmal Mahmoud Abdu Mohamed , _c2021
300			_a98 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology
520			_aBackground: C. psittacci is one of the most prevalent infections in aviculture and represents the most important animal chlamydiosis of zoonotic character. Purpose: To determine the most proper diagnostic tool for C. psittaci via comparing between different methods applied on this study, in addition to the molecular characterization of chlamydia psittaci isolates.Methodology: 233 serum samples, 330 fecal swabs and 736 internal organs (liver, lung, heart and spleen) were obtained from ducks, ostriches, quail and pigeons.Sixty four tracheal swabs were obtained fron ostrich. C. psittaci was isolated from the samples by Chicken embryo inoculation via yolk sac method. Impression smears were made from the collected yolk sac membranes, stained with Gimenez stain and examined under an oil immersion to detect the presence of chlamydial inclusions. Immunoperoxidase test was used for detection of C. psittaci inclusion bodies in the yolk sac impression smears. Complement fixation test was used for detection of antibodies in the serum samples. Then molecular characterization of chromosomal DNA of C. psittaci was performed by PCR. Results: Positive samples of isolation showed dwarfed and congested embryos with congestion of their yolk sac vessels. Immuno-peroxidase stain positive slides showed discrete, densely labeled brown inclusion bodies was seen with in transparent background. The PCR results of chlamydia psittaci isolates showed that, in fecal samples, ostrich species had positive percentage for chlamydia psittaci (91.7 %), pigeon (86.7 %), quail (85.7 %), duck (80 %). In the tracheal swabs, ostrich species were positive for chlamydia psittaci (83.3 %). Samples of each studied species were positive for the presence of 16S rRNA gene of chlamydia psittaci, and, out of 16 samples, 16 (100%) was positive for ompA
530			_aIssued also as CD
653		4	_aChlamydia psittaci
653		4	_aMolecular diagnosis
653		4	_aPsittacosis
700	0		_aHadia Abdelreheem Ali , _eSupervisor
700	0		_aKamelia Mahmoud Osman , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c82127 _d82127