000			03102cam a2200325 a 4500
003			EG-GiCUC
008			211020s2021 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.11.26.Ph.D.2021.Ge.S
100	0		_aGehad Ahmed Basuony Yousef
245	1	0	_aStudy on the potential effects of experimental blastocystis infection on lactase enzyme activity, histopathological, inflammatory and apoptotic changes in intestines of mice / _cGehad Ahmed Basuony Yousef ; Supervised Maha Mohammed Abou Elmagd Basyoni , Eman Ali Mohamed Mostafa , Mohamed Abbas Shemis
246	1	5	_aدراسة عن تاثير المحتمل لعدوى البلاستوسيستس التجريبيه على نشاط انزيم اللاكتيز والتغيرات المرضيه فى الانسجه ودلائل الالتهاب وموت الخلايا المبرمج فى امعاء الفئران
260			_aCairo : _bGehad Ahmed Basuony Yousef , _c2021
300			_a217 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Parasitology
520			_aThe present study was carried out on 20 laboratory bred male mice in Theodore Bilharz Research Institute to evaluate the influence of Blastocystis infection on lactase enzyme activity in the brush border of the intestine in experimentally infected mice and to detect the possible pathological changes, inflammatory and apoptotic markers provoked by Blastocystis infection. Assessment of lactase enzyme activity by lactase assay colorimetric kit on intestinal tissue and spectrophotometric measurements showed significant reduction of lactase enzyme in Blastocystis infected groups (Group II 4.593 ± 0.775 and Group IV 2.484± 0.908 with p values < 0.001) compared to the non-infected groups ( Group I 8.780 ± 0.263 and Group III 13.883 ±6.388 with p values< 0.001).In addition, histopathological examination of small intestinal tissue sections of infected mice showed moderate active inflammation with moderate number of vacuolar forms of Blastocystis while histopathological examination of large intestinal tissue sections showed severe active colitis with large number of vacuolar forms of Blastocystis.Furthermore, Immunohistochemical staining with tumor necrosis factor alpha (TNF-Ü) detected a significant elevation in Blastocystis infected groups compared to non-infected groups. Also, there is a significant elevation of proapoptotic marker BAX protein in Blastocystis infected groups and significant reduction of antiapoptotic BCL-2 in Blastocystis infected groups compared to the non-infected groups
530			_aIssued also as CD
653		4	_aBlastocystis
653		4	_aLactase
653		4	_aSpectrophotometer
700	0		_aEman Ali Mohamed Mostafa , _eSupervisor
700	0		_aMaha Mohammed Abou Elmagd Basyoni , _eSupervisor
700	0		_aMohamed Abbas Shemis , _eSupervisor
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c82713 _d82713