000			02955cam a2200325 a 4500
003			EG-GiCUC
008			211023s2021 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.12.05.Ph.D.2021.Am.A
100	0		_aAmira Abdrab Elnaby Mohamed Mazyad
245	1	0	_aAssessment of Ipomoea batatas (L.) Lam. viruses and their potential wild hosts in Egypt / _cAmira Abdrab Elnaby M. Mazyad ; Supervised Mahasen H. Ismail , Wafaa M. Amer , Amal Aboul Elela A. Farghaly
246	1	5	_aتقييم فيروسات البطاطا وعوائلها البرية فى مصر
260			_aCairo : _bAmira Abdrab Elnaby Mohamed Mazyad , _c2021
300			_a220 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Science - Department of Botany and Microbiology
520			_aGlobally, sweet potato (Ipomoea batatas (L.) Lam., family Convolvulaceae), is an important vegetable crop, unluckily, the viral infection induces a significant yield loss. The current study aims to identify viruses infecting sweet potato in Egypt and highlights the role of the interfacing weeds as viruses reservoir. The study covered the incidence of the six viruses (SPFMV, SPVG, SPLV, SPMMV, SPCSV and SPLCV) in different governorate in Egypt and focused on the two dominant potyviruses sweet potato feathery mottle virus (SPFMV) and sweet potato virus G (SPVG) in details both of transmission, host range, inclusion bodies, virus morphology, histopathology, serological diagnosis, genome identification using RT-PCR and multiplex RT-PCR. The results revealed that the potyviruses SPFMV and SPVG are the most devastating viruses affecting sweet potato in Egypt. Coinfection with of both viruses was the common feature in both of sweet potato and its interfacing weeds. A wider host range in families Convolvulaceae, Chenopodiaceae and Euphorbiaceae, was detected. The infection symptoms on sweet potato were comparable to that detected in the interfaced weeds. The infection-incidence in perennial Convolvulaceae and Tiliaceae weed-species showed 100% virus incidence. The characteristic amorphous inclusion bodies for the detected viruses and the cytopathological alterations on the host cells were observed under Transmission Electron Microscopy (TEM).The nucleotide sequences of the partial coat protein gene of the detected viruses were compared to the isolate available in GenBank. Next generation sequencing analysis for the complete genome sequence of the two SPFMV and SPVG viruses
530			_aIssued also as CD
653		4	_aIncidence
653		4	_aRT-PCR
653		4	_aSPFMV and SPVG ELISA
700	0		_aAmal Aboul Elela A. Farghaly , _eSupervisor
700	0		_aMahasen H. Ismail , _eSupervisor
700	0		_aWafaa M. Amer , _eSupervisor
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c82727 _d82727