000 02010cam a2200325 a 4500
003 EG-GiCUC
008 211024s2021 ua dh f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aPh.D
099 _aCai01.12.05.Ph.D.2021.Kh.E
100 0 _aKhaled Mohamed Elsaied
245 1 0 _aEffect of gamma radiation on bacterial redox protein azurin /
_cKhaled Mohamed Elsaied ; Supervised Doria Aly Zaki , Zahira Sayed Tawfik , Mourad M.S. Abulezz
246 1 5 _aأثر أشعة جاما على الريدوكسى بروتين البكتيرى - آزورين
260 _aCairo :
_bKhaled Mohamed Elsaied ,
_c2021
300 _a150 P. :
_bcharts , facsimiles ;
_c25cm
502 _aThesis (Ph.D.) - Cairo University - Faculty of Science - Department of Botany-Microbiology
520 _aThe D10 value of a clinical isolate of P. aeruginosa was calculated to be 0.12 kGy. The total protein-N contents of untreated and irradiated cells with {uF067}-irradiation up to 1.2 kGy declared a noticeable increase in the amount of protein contents in cells treated with 0.8 kGy (V1).The D10 value of (V1) was estimated to be 0.14 kGy. By repeating exposure of (V1) to Y-irradiation at 0.8 kGy (V2), (V3), (V4), (V5) and (V6) were induced. The maximum value of protein-N contents was estimated in (V3). The protein profile from SDS-PAGE analysis indicated that the maximum percentage of azurin was recorded in (V3). The amino acid profile and functional groups of purified azurin from (V3) and the standard azurin revealing the similarity between the extracted and the standard azurin
530 _aIssued also as CD
653 4 _aProtein conten
653 4 _aPseudomonas aeruginosat
653 4 _aY-radiation, , D10
700 0 _aDoria Aly Zaki ,
_eSupervisor
700 0 _aMourad M. S. Abulezz ,
_eSupervisor
700 0 _aZahira Sayed Tawfik ,
_eSupervisor
905 _aNazla
_eRevisor
905 _aShimaa
_eCataloger
942 _2ddc
_cTH
999 _c82789
_d82789