000			02716cam a2200325 a 4500
003			EG-GiCUC
008			211225s2021 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.10.14.Ph.D.2021.Es.C
100	0		_aEsraa Aly Sayed Ismail
245	1	0	_aCryoprotectants and gene expression of cryopreserved oocytes and embryos in buffalo / _cEsraa Aly Sayed Ismail ; Supervised Mohamed A. I. Elsayed , Nabil A. Hemeida , Omaima M. Kandil
246	1	5	_aعوامل الوقاية من التجميد والتعبير الجينى لبويضات وأجنة الجاموس المحفوظة بالتجميد
260			_aCairo : _bEsraa Aly Sayed Ismail , _c2021
300			_a120 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Theriogenology
520			_aThis work was carried out for four successive years (2018, 2019 , 2020 and 2021) in order to investigate the effect of different cryoprotectant agents on 1) vitrified/thawed buffalo oocytes developmental competence, 2) viability of vitrified/thawed transferable buffalo embryos and 3) gene expression of in vitro vitrified/thawed matured buffalo oocytes and embryos. Excellent and good oocytes were cultured in TCM-199 medium at 38.5 {u00BA}C, 5% CO2 for 22 hrs, then fertilized using frozen semen and cultured on SOF media. Matured oocytes or transferable embryos were vitrified in DMSO, EG or combination of DMSO+EG by two steps procedure and stored in LN. The morphologically normal vitrified/thawed in vitro matured oocytes were fertilized using frozen semen and cultured on SOF media for detection the cleavage and transferable embryos rates to assess its viability. Fresh matured buffalo oocytes/embryos and recovered vitrified/thawed buffalo oocytes/ embryos were stained with Hochest stain to assess the stage of maturation of in vitro matured oocytes or cell count of in vitro produced embryos and stained with Mitotricker red for detection the mitochondrial distribution and intensity. mRNA was extracted from vitrified/thawed buffalo in vitro matured oocytes or in vitro produced embryos vs fresh matured oocytes or embryos using Picopure kits, cDNA was synthesized by QuantiTect Reverse Transcription kits
530			_aIssued also as CD
653		4	_aBuffalo
653		4	_aIn Vitro Embryo Production
653		4	_aVitrification
700	0		_aMohamed A. I. Elsayed , _eSupervisor
700	0		_aNabil A. Hemeida , _eSupervisor
700	0		_aOmaima M. Kandil , _eSupervisor
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c83680 _d83680