000			03051cam a2200325 a 4500
003			EG-GiCUC
008			211227s2021 ua d f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.10.14.Ph.D.2021.Ya.I
100	0		_aYasser Hussein Ali Saber
245	1	0	_aImproving viability and cryotolerance of in vitro produced cattle embryos / _cYasser Hussein Ali Saber ; Supervised Adel A. M. Seida , Refaat S. A. Ragab , Wahid M. M. Ahmed
246	1	5	_aتحسين حيوية وتحمل التجميد لأجنة الأبقار المنتجة معمليا
260			_aCairo : _bYasser Hussein Ali Saber , _c2021
300			_a118 P. : _bcharts ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Theriogenology
520			_aIn vitro embryo production (IVEP) is considered as one of the most important technologies in cattle industry due to production of superior quality embryos with high developmental competence that use at low costs in embryo transfer programs. So, improvement of in vitro culture systems are the most critical and essential step to keep on this biotechnology. Hence, the current experiments were designed. Experiment 1, aimed to (I) study the effect of phenazine ethosulfate (PES) supplementation in culture media on the selected miRNAs (miR-205, miR-26a-5p, and let-7b) and their target genes (OCT4, DNMT, CASP3, ATF6, ATP5ME, and ELOVL5), during bovine embryo production. Therefore, a group of two-day bovine embryos was cultured in a medium with lipid-reducing agent, PES (0.3 mM). Another group of embryos without PES was left as a control. Embryos were vitrified and morphologically examined after warming and the viability was evaluated by culturing for 24 h. After evaluation, embryos were classified as good or poor. Afterwards, embryos (blastocyst and morula) were kept at -80{u00B0}C for RNA extraction and qRT-PCR of miRNAs and their targets. Results revealed that the rate of morula was higher (P<0.01) in treated compared to control groups. After vitrifications, the percentage of good quality embryos increased in treated than control groups. Additionally, the rate of dead embryos was high in control groups.The Let-7b and miR-205 were significantly over-expressed in the treated good as well as poor embryos compared to control (untreated) good and poor embryos, respectively. However, miR-26 was suppressed in the treated good and poor embryos compared to control (untreated) embryos, respectively. Both of OCT4and DNMT1 transcripts up-regulated in the treated (good& poor) embryos compared to control groups
530			_aIssued also as CD
653		4	_aBovine
653		4	_aGene expression
653		4	_aPhenazine ethosulfate
700	0		_aAdel A. M. Seida , _eSupervisor
700	0		_aRefaat S. A. Ragab , _eSupervisor
700	0		_aWahid M. M. Ahmed , _eSupervisor
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c83737 _d83737