000			03023cam a2200349 a 4500
003			EG-GiCUC
005			20250223032908.0
008			220103s2021 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.10.17.M.Sc.2021.Kh.C
100	0		_aKhaled Abdelaziz Mohammed Abdelaziz
245	1	0	_aComparative molecular studies on FMD virus in some Egyptian governorates / _cKhaled Abdelaziz Mohammed Abdelaziz ; Supervised Ahmed Abdelghani Elsanousi , Ayman Hanae Eldeeb , Momtaz Abdelhady Shahein
246	1	5	_aدراسات جزيئية مقارنة على فيروس مرض الحمى القلاعية فى بعض محافظات مصر
260			_aCairo : _bKhaled Abdelaziz Mohammed Abdelaziz , _c2021
300			_a158 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology
520			_aFoot and mouth disease (FMD) is a cross-border Transmissible viral disease affecting a wide variety of animals. Egypt is under the potential risk of infection from neighboring countries with a background of the disease epidemics from 1950 till now although a comprehensive vaccination programme has been adopted. The present study aims to make a molecular comparison between foot and mouth disease virus in some Egyptian governorates,14 FMD suspected samples were collected from diseased animals in the period from 2016 to 2019, the samples were FMD positive by Antigen detection ELISA, subjected for trials of FMDV isolation on the BHK-21 cell line, RT-PCR serotyping, and VP1 sequencing.VP1 encoding region sequencing and phylogenetic analysis were performed for some representative samples for each serotype. Antigen detection serotyping ELISA results were three samples positive for A serotypes, three for serotype O, and eight for serotype SAT2. The isolation attempt was successful for the A and O serotypes on the BHK-21 cell line. RT-PCR confirmed the ELISA results for both samples and tissue culture harvest. VP1 sequencing and phylogenetic analysis revealed that all serotype O strain belonged to topotype East Africa 3(EA-3) and were identical to each other with a percentage of 91 to 94%, while serotype A belonged to topotype Africa, lineage G-IV with 97 to 99 identity to each other, and serotype SAT2 samples were identical to each other with a percentage of 99% and were related with a percentage of 96% identity to topotype VII, Lib-12 a lineage that varies from previously recorded Egyptian Ghb-12 lineage G-VII strains
530			_aIssued also as CD
653		4	_aELISA
653		4	_aFMDV
653		4	_aRT-PCR
700	0		_aAhmed Abdelghani Elsanousi , _eSupervisor
700	0		_aAyman Hanae Eldeeb , _eSupervisor
700	0		_aMomtaz Abdelhady Shahein , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c83841 _d83841