000 | 03310cam a2200349 a 4500 | ||
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003 | EG-GiCUC | ||
005 | 20250223032915.0 | ||
008 | 220130s2021 ua do f m 000 0 eng d | ||
040 |
_aEG-GiCUC _beng _cEG-GiCUC |
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041 | 0 | _aeng | |
049 | _aDeposite | ||
097 | _aM.Sc | ||
099 | _aCai01.07.06.M.Sc.2021.Al.E | ||
100 | 0 | _aAlshimaa Ibrahem Ahmed | |
245 | 1 | 0 |
_aEvaluation of probiotic functional Properties of some egyptian lactic acid bacteria and yeast isolates / _cAlshimaa Ibrahem Ahmed ; Supervised Galal Mahmoud Khalafallah , Hanan Mohamed Abdellatif Goda , Gihan Mohamed Elmoghazy |
246 | 1 | 5 | _aتقييم خصائص البروبيوتك الوظيفية لبعض العزلات المصرية من بكتيريا حامض اللاكتيك و الخمائر |
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_aCairo : _bAlshimaa Ibrahem Ahmed , _c2021 |
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300 |
_a85 P . : _bcharts , photographs ; _c25cm |
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502 | _aThesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Microbiology | ||
520 | _aThis study is the first one to isolate Enterococcus mediterraneensis in Egypt and to characterize it as new species of probiotics globally. Fourteen LAB isolates, from different dairy and meat products, were identified as Enterococcus mediterraneensis, Lactobacillus fermentum and Streptococcus lutetiensis by 16S rRNA gene sequencing, and in vitro characterized for their actual probiotic potential. All Ent. mediterraneensis isolates were resistant to clindamycin, whereas Lb. fermentum isolates were resistant to ampicillin, clindamycin and vancomycin. The Ent. mediterraneensis and Lb. fermentum isolates displayed high overall digestive survival ranged from 1.35±0.06 to 32.73±0.84% and from 2.01±0.01 to 23.9±1.85%, respectively. All isolates displayed cell surface hydrophobicity ranged between 15.44±6.72 and 39.79±2.87%. The strongest auto-aggregation capability, higher than 40% after 24h, was observed for most Ent. mediterraneensis and Lb. fermentum isolates. The Ent. mediterraneensis isolates (L12 and L15), Lb. fermentum (L8, L9 and L10) and S. lutetiensis (L14) exhibited the greatest co-aggregation with Sal. typhimurium, E. coli O157:H7, Lis. monocytogenes, Staph. aureus and B. cereus. The results of antimicrobial assay confirmed that, 57.14% of Ent. mediterraneensis isolates could be considered bacteriocinogenic against E. coli O157: H7, B. cereus and Staph. aureus. For Lb. fermentum, 60% were bacteriocinogenic isolates against Sal. typhimurium and Staph. aureus. For Lis. monocytogenes, 42.86 and 40% of Ent. mediterraneensis and Lb. fermentum isolates, respectively were bacteriocinogenic. According to the results, Ent. mediterraneensis (L2, L12 and L15), Lb. fermentum (L8, L9 and L10) and S. lutetiensis (L14) are the most promising in vitro probiotic candidates, and require in vivo assessment | ||
530 | _aIssued also as CD | ||
653 | 4 | _aEnterococcus mediterraneensis | |
653 | 4 | _aLactobacillus fermentum | |
653 | 4 | _aStreptococcus lutetiensis | |
700 | 0 |
_aGalal Mahmoud Khalafallah , _eSupervising |
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700 | 0 |
_aGihan Mohamed Elmoghazy , _eSupervising |
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700 | 0 |
_aHanan Mohamed Abdellatif Goda , _eSupervising |
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856 | _uhttp://172.23.153.220/th.pdf | ||
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_aAmira _eCataloger |
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905 |
_aNazla _eRevisor |
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_2ddc _cTH |
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_c84061 _d84061 |