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040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.07.06.M.Sc.2021.Al.E
100 0 _aAlshimaa Ibrahem Ahmed
245 1 0 _aEvaluation of probiotic functional Properties of some egyptian lactic acid bacteria and yeast isolates /
_cAlshimaa Ibrahem Ahmed ; Supervised Galal Mahmoud Khalafallah , Hanan Mohamed Abdellatif Goda , Gihan Mohamed Elmoghazy
246 1 5 _aتقييم خصائص البروبيوتك الوظيفية لبعض العزلات المصرية من بكتيريا حامض اللاكتيك و الخمائر
260 _aCairo :
_bAlshimaa Ibrahem Ahmed ,
_c2021
300 _a85 P . :
_bcharts , photographs ;
_c25cm
502 _aThesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Microbiology
520 _aThis study is the first one to isolate Enterococcus mediterraneensis in Egypt and to characterize it as new species of probiotics globally. Fourteen LAB isolates, from different dairy and meat products, were identified as Enterococcus mediterraneensis, Lactobacillus fermentum and Streptococcus lutetiensis by 16S rRNA gene sequencing, and in vitro characterized for their actual probiotic potential. All Ent. mediterraneensis isolates were resistant to clindamycin, whereas Lb. fermentum isolates were resistant to ampicillin, clindamycin and vancomycin. The Ent. mediterraneensis and Lb. fermentum isolates displayed high overall digestive survival ranged from 1.35±0.06 to 32.73±0.84% and from 2.01±0.01 to 23.9±1.85%, respectively. All isolates displayed cell surface hydrophobicity ranged between 15.44±6.72 and 39.79±2.87%. The strongest auto-aggregation capability, higher than 40% after 24h, was observed for most Ent. mediterraneensis and Lb. fermentum isolates. The Ent. mediterraneensis isolates (L12 and L15), Lb. fermentum (L8, L9 and L10) and S. lutetiensis (L14) exhibited the greatest co-aggregation with Sal. typhimurium, E. coli O157:H7, Lis. monocytogenes, Staph. aureus and B. cereus. The results of antimicrobial assay confirmed that, 57.14% of Ent. mediterraneensis isolates could be considered bacteriocinogenic against E. coli O157: H7, B. cereus and Staph. aureus. For Lb. fermentum, 60% were bacteriocinogenic isolates against Sal. typhimurium and Staph. aureus. For Lis. monocytogenes, 42.86 and 40% of Ent. mediterraneensis and Lb. fermentum isolates, respectively were bacteriocinogenic. According to the results, Ent. mediterraneensis (L2, L12 and L15), Lb. fermentum (L8, L9 and L10) and S. lutetiensis (L14) are the most promising in vitro probiotic candidates, and require in vivo assessment
530 _aIssued also as CD
653 4 _aEnterococcus mediterraneensis
653 4 _aLactobacillus fermentum
653 4 _aStreptococcus lutetiensis
700 0 _aGalal Mahmoud Khalafallah ,
_eSupervising
700 0 _aGihan Mohamed Elmoghazy ,
_eSupervising
700 0 _aHanan Mohamed Abdellatif Goda ,
_eSupervising
856 _uhttp://172.23.153.220/th.pdf
905 _aAmira
_eCataloger
905 _aNazla
_eRevisor
942 _2ddc
_cTH
999 _c84061
_d84061