| 000 | 02744cam a2200349 a 4500 | ||
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| 003 | EG-GiCUC | ||
| 005 | 20250223032919.0 | ||
| 008 | 220212s2021 ua d f m 000 0 eng d | ||
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_aEG-GiCUC _beng _cEG-GiCUC |
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| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aM.Sc | ||
| 099 | _aCai01.12.25.M.Sc.2021.He.A | ||
| 100 | 0 | _aHebatallah Gamal Hafez Abdelhameed | |
| 245 | 1 | 0 |
_aAssessment of potential anti-cancer stem cell activity of some natural compounds in breast cancer cells in vitro / _cHebatallah Gamal Hafez Abdelhameed ; Supervised Rafat Milad Mohareb , Emad Fawzy Eskander |
| 246 | 1 | 5 | _aتقدير كفاءة بعض المركبات الطبيعية كمضادات للخلايا الجذعية السرطانية فى خلايا سرطان الثدى خارج جسم الكائن الحى |
| 260 |
_aCairo : _bHebatallah Gamal Hafez Abdelhameed , _c2021 |
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| 300 |
_a125 P. : _bcharts ; _c25cm |
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| 502 | _aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biotechnology | ||
| 520 | _aThis study aimed to appraise the activity of Jania rubens, Petrocladia capillacea, Corallina officinalis, Colpomenia sinuosa, Sargassum asperifolium and Sargassum dentifolium polysaccharides against breast cancer stem cells (BCSCs) in MDA-MB-231 and MCF-7 cell lines. These compounds were characterized to be sulfated polysaccharide-protein complexes. Cytotoxicity of the polysaccharides against MDA-MB-231, MCF-7 breast cancer cell lines and normal Vero E6 cells was assessed by MTT (3-(4,5-dimethylthiazol-2-yl)- 2,5- diphenyltetrazolium bromide) assay, and their impact on CD44+/CD24{u2212} and aldehyde dehydrogenase 1(ALDH1) positive BCSC population was determined by flow cytometry. Their effect on the gene expression of CSC markers, Wnt/Ý- catenin and Notch signaling pathways was evaluated by quantitative real-time PCR. All tested polysaccharides inhibited the growth of breast cancer cells with a more prominent action against MDA-MB-231 than MCF-7 cells at most time intervals along with their weaker cytotoxicity against Vero E6 cells, and reduced BCSC subpopulation. P. capillacea polysaccharides down-regulated the expression levels of OCT4, SOX2 and ALDH1A3 genes in MDA-MB-231 as well as in MCF-7 cells. C. officinalis polysaccharides exhibited similar effects on SOX2 and ALDH1A3 genes | ||
| 530 | _aIssued also as CD | ||
| 650 | 0 | _aAnti-cancer | |
| 653 | 4 | _aBreast cancer | |
| 653 | 4 | _aNtural compounds | |
| 653 | 4 | _aPotential anti-cancer stem | |
| 700 | 0 |
_aEmad Fawzy Eskander , _eSupervisor |
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| 700 | 0 |
_aRafat Milad Mohareb , _eSupervisor |
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| 856 | _uhttp://172.23.153.220/th.pdf | ||
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_aNazla _eRevisor |
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_aShimaa _eCataloger |
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_2ddc _cTH |
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| 999 |
_c84179 _d84179 |
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