| 000 | 03273cam a2200361 a 4500 | ||
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| 003 | EG-GiCUC | ||
| 005 | 20250223032921.0 | ||
| 008 | 220217s2021 ua dh f m 000 0 eng d | ||
| 040 |
_aEG-GiCUC _beng _cEG-GiCUC |
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| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aM.Sc | ||
| 099 | _aCai01.07.03.M.Sc.2021.Sh.E | ||
| 100 | 0 | _aSherif Mohamed Abdelhameed Omara | |
| 245 | 1 | 0 |
_aEffects of curcumin supplementation on viability and Antioxidant capacity of buffalo granulosa cells Under in vitro culture conditions / _cSherif Mohamed Abdelhameed Omara ; Supervised Nasser Ghanem Othman , Marwa Said Faheem , Ayman Moustafa Saeed |
| 246 | 1 | 5 | _aتأث{u٠٦أأ}رات إضافة الكركم على ح{u٠٦أأ}و{u٠٦أأ}ة والنشاط المضاد للأكسدة لخلا{u٠٦أأ}ا الجاموس الحب{u٠٦أأ}ب{u٠٦أأ}ة التى تم تنم{u٠٦أأ}تھا معمل{u٠٦أأ}ا |
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_aCairo : _bSherif Mohamed Abdelhameed Omara , _c2021 |
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| 300 |
_a92 P . : _bcharts , facsmilies ; _c25cm |
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| 502 | _aThesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Animal Production | ||
| 520 | _aThe current study was conducted to investigate the possible protective effects of curcumin supplementation on buffalo granulosa cells (GCs) under in vitro culture conditions. Buffalo ovaries were collected from local abattoir in physiological saline solution at 37oc and transported directly to laboratory. Follicular fluid containing GCs and cumulus-oocyte-complexes were aspirated from antral follicles with diameter 2-8 mm. The collected GCs were seeded (Approximately 375,000 viable cells) in a 8-well culture plate containing 199-tissue culture medium (TCM) and kept at 37{u00B0}C in a humidified atmosphere of 5% CO2. The curcumin was supplemented to TCM media at levels of 1, 2.5, 5 and 10 oM or kept without treatment as control group for 48 hours. The viability of cells was determined using the trypan blue test. Intracellular reactive oxygen species (ROS) level was assessed by measuring the fluorescent intensity of 6-carboxy-2{u2032}, 7{u2032}-dichlorodihydro fluorescein diacetate (H2DCFDA). In addition, mitochondrial activity of GCs was using Mitrocker Red Fluorescent stain. The results of the present study indicated that the viabilityof GCsunder culture conditions were significantly decreased p {u2264} 0.05 in groups treated with 1, 2.5, 5 and 10 oM curcumin (86.0%, 86.26%, 83.0% and 74.0%, respectively) compared to control group (93.60%).The two groups of granulosa cells cultured with 2.5 and 5 oM curcumin recorded greater levels of mitochondrial activity than the groups cultured with 1 oM and 10 oM curcumin. In addition, there was a significant increase in ROS level in group cultured with 10oM curcumin, compared to control and other experimental groups | ||
| 530 | _aIssued also as CD | ||
| 650 | 0 | _aAnimal production | |
| 653 | 4 | _aAntioxidant | |
| 653 | 4 | _aBuffalo | |
| 653 | 4 | _aGranulosa cells | |
| 700 | 0 |
_aAyman Moustafa Saeed , _eSupervising |
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| 700 | 0 |
_aMarwa Said Faheem , _eSupervising |
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| 700 | 0 |
_aNasser Ghanem Othman , _eSupervising |
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| 856 | _uhttp://172.23.153.220/th.pdf | ||
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_aAmira _eCataloger |
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| 905 |
_aNazla _eRevisor |
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_2ddc _cTH |
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_c84249 _d84249 |
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