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040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aPh.D
099 _aCai01.07.05.Ph.D.201.Sa.B
100 0 _aSally Farag Desouky Mohamed
245 1 0 _aBotanical and biotechnological studies of asparagus plant (asparagus officinalis l.) under the influence of nano zinc /
_cSally Farag Desouky Mohamed ; Supervised Atef Zakaria Elsayed Sabh , Zeinab Kassem Taha , Hattem Mohamed Elshabrawi
246 1 5 _aتحت تأثير النانو زنك (Asparagus officinalisL.) دراسات نباتية وبيوتكنولوجية على نبات الأسبرجس
260 _aCairo :
_bSally Farag Desouky Mohamed ,
_c2021
300 _a166 P. :
_bcharts , facsimiles ;
_c25cm
502 _aThesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Agr. Botany
520 _aThe current study was performed on Asparagus officinalisL. (Mary Washington 500 W cultivar) of the family Asparagaceae, during the successive seasons 2017 {u2013} 2019 through three experiments; the 1st one goaled to display the effect of Zinc Oxide nanoparticles (ZnONPs) onAsparagus officinalis L. plant by using some morphological, histological, and biochemical parameters. Seeds were soaked in different concentrations of ZnO nanoparticles (0.5, 1, 3, 5, 10, 30, 50, 100, and 500 ppm) for 48h, in addition to Zn acetate Bulk (40 ppm) treatment and untreated control for comparison. The obtained results indicated that ZnO NPs treatment at the concentration of 500 ppm showed the highest values of all studied morphological characters of Asparagus plant with the exception of root length. In addition, a significant increase in catalase enzyme activity was observed when using a concentration of 500 ppm compared to the other treatments. The concentration of 3 ppm led to the highest stimulation effect for both types of flavonoids (Rutin and Myricetin), and the highest Naringenin and total flavonoids contents were detected when 5 ppm of ZnO NPs was used. With regard to the effect of Nano-Zinc on the anatomical features of the asparagus plant, it was found that there was a clear effect on the anatomical characteristics of the plant (root and stem) treated with a concentration of 500 ppm of ZnO NPs compared to plant treated with Zinc acetate at 40 ppm and untreated control plant.The2nd experiment (in vitro)aimed to evaluate the effect of Zinc Oxide nanoparticles (ZnO NPs) at different concentrationson callus biomass, cell suspensionviability (%) and flavonoids content in callus and suspension cultures of Asparagus plant. The results clarified that the treatment 10 ppm of ZnO NPs had the greatest callus fresh weight (8.01g) with a significant increase of 352.5% related to untreated control. The highest cell viability percentage wasrecorded at 10 ppm ofZnO NPs concentration on the 9th day. In callus culture, using ZnO NPs decreased the callus content of Rutin and total flavonoids compared with control. In suspension culture, the best flavonoid detected was Rutin compound (37.78 mg/100g) under10 ppm of ZnO NPs concentration with the third rank of total flavonoids content (94.59 mg/100g). The 3rd experiment (Antimicrobial effects)showed that the biomass and solvent types have an important role in inhibitingmicrobial activity.Root extracts were more effective than shoot extracts in most cases
530 _aIssued also as CD
650 0 _aBotany
653 _aAsparagus
653 _aGrowth parameters
653 _aZinc Oxide nanoparticles
700 0 _aAtef Zakaria Elsayed Sabh ,
_eSupervisor
700 0 _aHattem Mohamed Elshabrawi ,
_eSupervisor
700 0 _aZeinab Kassem Taha ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aShimaa
_eCataloger
942 _2ddc
_cTH
999 _c84447
_d84447