Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Pathology

The aim of this work is to study the immunohistochemical expression of CD10 in urothelial carcinoma of the urinary bladder and its possible prognostic value. Forty one radical cystectomy specimens; collected from the pathology department, Faculty of Medicine, Cairo University. Two slides were prepared from each paraffin embedded tumor block, one slide was stained for Hematoxylin and eosin (H and E) staining for histopathological reassessment and the other positively charged slide was stained immunohistochemically by CD10 monoclonal antibody. The immunohistochemical demonstration of the immunohistochemical marker was accomplished by using immunoperoxidase method. Staining of the cell membrane and/or cytoplasm was considered positive expression. A semi - quantitative scoring based on the percentage of positive cells was performed. Positive CD10 immunohistochemical expression was found to be significantly correlated with patients' age, tumor size, presence of squamoid differentiation and positive lymph node metastasis. Also there was significant correlation between CD10 scoring and presence of extravesical extension as well as vascular invasion. No significant correlation between CD10 immunohistochemical expression and tumor stage could be detected

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