Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology

The effectiveness of many antibiotics has been decreased strongly due to bacterial resistance, so the aim the present work is to explore a novel antimicrobial nanocomposite with greater efficacy against resistant bacteria.For this purpose, a total of 49 isolates were recovered from blood, wound, burn, sputum and urine specimens collected from different hospitals in Egypt: 8 isolates (16.4%) were identified as Staphylococcus aureus, 14 isolates (28.5%) were identified as Pseudomonas aeruginosa, 15 isolates (30.6%) were identified as Klebsiella pneumonia, 9 (18.3 %) isolates were identified as Acinetobacter baumannii and 3 isolates (6.2 %) were identified as Proteus mirabilis. The antibiotic susceptibility of the isolates against different classes of antibiotics was then carried out. The majority of the clinical isolates showed multidrug resistance profiles.Tobramycin and levofloxacin were the two antibiotics to which the majority of clinical isolates were resistant. They were therefore selected for conjugation with silver nanoparticles with the aim to increase their antimicrobial activity and overcome/reduce the resistance of the clinical isolates.Tobramycin- and levofloxacin-silver nanocomposites were synthesized and characterized by Transmission Electron Microscopy, UV-VIS Spectroscopy, Zetasizer and Fourier Transform Infrared spectroscopy

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