Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology

As the labeled anti-camel Igs with enzymes for ELISA are unavailable in commercial level, the present investigation was directed for developing labeled anti-camel IgG with horseradish peroxidase. For purification of camel IgG whole molecule, camel sera were preliminary precipitated with 50% saturated ammonium sulphate and dialyzed against diluted PBS then concentrated. This preparation was followed by Protein A Sepharose affinity column chromatograhy for purification of camel IgG. The purity of the eluted camel IgG was tested by SDS-PAGE. Four bands of molecular weight 63, 52, 40 and 33 kDa, represent camel IgG, were detected. Anti-camel IgG was prepared by immunization of goats and rabbits separately, with purified camel IgG. Then anti-camel IgG was purified by loading on Protein A Sepharose affinity column chromatography after precipitation and concentration. Whole molecule anti-camel IgG was conjugated with horseradish peroxidase

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