Full sequence analysis of F gene of NDV and comparative evaluation of genotype II and VII vaccines / Karim Mohamed Selim ; Supervised Ahmed Abdelghani Elsanousi , Hussein Aly Hussein Ahmed , Abdelsatar Arafa Mohamed

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Karim Mohamed Selim

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TextLanguage: English Publication details: Cairo : Karim Mohamed Selim , 2018Description: 180 P. : charts ; 25cmOther title:

VII و II لفيروس مرض النيوكاسل والتقيم المقارن للقاحات النوع الجيني رقم F تحليل التتابع النيوكلوتيدي الكامل لجين [Added title page title]

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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology Summary: The present study was conducted using samples from seven suspected Newcastle disease virus (NDV) flocks of vaccinated chickens during 2012 to 2016 from 6 governorates in Egypt. The pathogenicity of the NDV isolates has been estimated through ICPI and ranged from 1.66 to 1.73 which indicates the velogenic type of NDV isolates. Pathotyping and genotyping of these isolates were done through sequencing of full length F gene. Results indicated that the seven NDV isolates showed characteristic cleavage site motif (112RRQKRF117) for the velogenic strains of NDV. Phylogenetic analysis of the F gene clustered these isolates within group I of genotype VIId within Israeli strains NDV/IS/2015, NDV-Ch/SD883, and most of Middle East strains. Six out of seven sequenced isolates have 6 potential N- linked glycosylation sites. The neutralization epitope on the 5 antigenic sites of fusion are conserved in all Egyptian strains of this study except NDV- KFR-B7-2012 which has a substitution at D170 G in epitope A4. In this study we compare two vaccination programs one using genotype VII inactivated and live NDV vaccine and second using genotype II inactivated and live vaccine. Contact chicks were added in both groups post challenge

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Item type	Current library	Home library	Call number	Copy number	Status	Barcode
Thesis	قاعة الرسائل الجامعية - الدور الاول	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.10.17.Ph.D.2018.Ka.F (Browse shelf(Opens below))		Not for loan	01010110077640000
CD - Rom	مخـــزن الرســائل الجـــامعية - البدروم	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.10.17.Ph.D.2018.Ka.F (Browse shelf(Opens below))	77640.CD	Not for loan	01020110077640000

Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology

The present study was conducted using samples from seven suspected Newcastle disease virus (NDV) flocks of vaccinated chickens during 2012 to 2016 from 6 governorates in Egypt. The pathogenicity of the NDV isolates has been estimated through ICPI and ranged from 1.66 to 1.73 which indicates the velogenic type of NDV isolates. Pathotyping and genotyping of these isolates were done through sequencing of full length F gene. Results indicated that the seven NDV isolates showed characteristic cleavage site motif (112RRQKRF117) for the velogenic strains of NDV. Phylogenetic analysis of the F gene clustered these isolates within group I of genotype VIId within Israeli strains NDV/IS/2015, NDV-Ch/SD883, and most of Middle East strains. Six out of seven sequenced isolates have 6 potential N- linked glycosylation sites. The neutralization epitope on the 5 antigenic sites of fusion are conserved in all Egyptian strains of this study except NDV- KFR-B7-2012 which has a substitution at D170 G in epitope A4. In this study we compare two vaccination programs one using genotype VII inactivated and live NDV vaccine and second using genotype II inactivated and live vaccine. Contact chicks were added in both groups post challenge

Issued also as CD

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