Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology

Campylobacter is a worldwide infection, which has been estimated as the most significant economic burden by EFSA and ECDC in 2016. It caused mainly by either Campylobacter jejuni or Campylobacter coli. Campylobacter species are one of the main cause of gastroenteritis for humans in developed and developing countries. The disease is endemic in Egypt and is a major cause of diarrhea in children. Good understanding of epidemiology and surveillance of Campylobacter will help in the elimination and prevention of it among animals and humans. Several intervention strategies have been implemented to reduce the intestinal colonization in chicken and vaccination measures is one of the effective control methods. The main goal of the present study was to develop a vaccine for reducing the intestinal burden of Campylobacter in chickens. To achieve this, 290 samples were collected from broiler flocks and slaughter market from Cairo governorate, Egypt. Vaccination of chickens with a prepared killed whole-cell vaccine was carried out.The Campylobacter specific IgG were measured in collected serum samples using ELISA assay. Also, Campylobacter count in the cecal content and serum antibody which have been measured. The greatest reduction in C.jejuni colonization was determined. Production of Campylobacter specific IgG antibodies as well as a marked decrease in C.jejuni colonization were recorded in chickens. In conclusion, we approved that the vaccination with CWC provides 73.3% protection of chickens from C.jejuni colonization

Issued also as CD