Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Analytical Chemistry

Two developed electrochemical biosensors were constructed for potentiometric determination of Ivabradine hydrochloride (IVB) in physiological fluids. The carbon-paste electrodes (CPEs) were constructed using nitrophenyl octyl ether as a plasticizer, graphite as source of carbon, Fe₂O₃ nanoparticles decorated multiwalled carbon nanotubes (Fe₂O₃@MWCNTs) as a modifier, t-butyl calixarene (t-BCX) or tailored molecularlyimprinted polymer (MIP) as ionophores. Loading the Fe2O3@MWCNTs into the CPEs increases the sensitivity and detection limit of the biosensors comparing with loading unmodified MWCNTs. MIP- and t-BCX-based CPEs exhibit Nernstian response of 56.0 and 58.9 mV decade-1 over drug concentration ranges of 10-3-10-8 and 10-3-10-7 M with detection limits of 9.8{u00D7}10-8 and 3.6{u00D7}10-8 M, respectively. Cavities in MIP and t-BCX are suitable for IVB masking with different complexing strengths, which were estimated by the sandwich membrane method (SMM). The developed biosensors exhibit better selectivity and higher formation constant because the cavities are tailored and more fitted for IVB. The developed biosensors are Sensitive for IVB determination in urine, serum and pharmaceutical preparations (Savapran®)

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