Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine- Department of Microbiology

The main objective of the present work was to produce IgY specific to Helicobacter pylori (H. pylori), the bacteria of peptic ulcer, chronic gastritis and gastric cancers. The produced antibodies are to be further employed for diagnostic (preparation of conjugates to help in the diagnosis of helicobacter infections in human and animal) or therapeutic purposes.Polyclonal anti-H. pylori IgY antibodies were successfully produced, purified, and titrated in this study.This was accomplished through immunization of laying hens with the bacterial cell lysate and isolation of the anti-H. pylori IgY by ammonium sulphate precipitation and affinity chromatography. Freund{u2019}s adjuvants were used with the immunogen.The purified IgY was proved to be reactive with the bacterial cells with high titer as indicated by ELISA and microplate agglutination. This article comes in accordance with the European Centre for the Validation of Alternative Methods (ECVAM) concerning alternative methods of value to the bioscience and medical fields that call for refining or replacing the use of laboratory animal

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