The effect of elevated concentration of soil carbon dioxide with partial root zone drying on the growth rate, yield, water productivity, and fruit quality of cucumber under greenhouse conditions /
Mohamed Sayed Mohamed Nageh Ali,
The effect of elevated concentration of soil carbon dioxide with partial root zone drying on the growth rate, yield, water productivity, and fruit quality of cucumber under greenhouse conditions / تأثير زيادة تركيز ثاني أكسيد الكربون في التربة مع التجفيف الجزئي لمنطقة الجذر على معدل النمو، المحصول، إنتاجية المياه، وجودة ثمار الخيار تحت ظروف مناخ الصوبات الزراعية by Mohamed Sayed Mohamed Nageh Ali ; Supervisors Dr. Hassan Ali Hassan, Dr. Emad Abdelhameed Abdeldaym, Dr. Mohamed EL-Sayed Abuarab. - 130 pages : illustrations ; 25 cm. + CD.
Thesis (M.Sc)-Cairo University, 2025.
Bibliography: pages 110-130.
Water scarcity and soil carbon dioxide elevation in arid regions are considered the most serious
factors affecting crop growth and productivity. This study aimed to investigate the impacts of
elevated CO2 levels (eCO2 at rates of 700 and 1000 ppm) on agro-physiological attributes to
induce drought tolerance in cucumbers by activating the expression of genes related to
aquaporin and stress response, which improved the yield of cucumber under two levels of
irrigation water conditions [75% and 100% crop evapotranspiration (ETc)]. Therefore, two field
experiments were conducted in a greenhouse with controlled internal climate conditions, at the
Mohamed Naguib sector of the national company for protected agriculture, during the winter
seasons of 2021-2022 and 2022-2023. The treatments included eCO2 in soil under normal and
partial root zoon drying (PRD, 100% ETc Full irrigations, and 75% ETc). All the applied
treatments were organized as a randomized complete block design (RCBD) and each treatment
was replicated six times. Untreated plants were designed as control treatment (CO2
concentration was 400 ppm). The results of this study showed: that elevating CO2 at 700 and
1000 ppm in soil significantly increased plant growth parameters, photosynthesis
measurements, and phytohormones [indole acetic acid (IAA) and gibberellic acid (GA3)], under
partial root-zone drying (75% ETc) and full irrigation conditions (100% ETc). Under PRD
condition, eCO2 at 700 ppm significantly improved plant height (13.68%), number of shoots
(19.88%), Leaf greenness index (SPAD value, 16.60%), root length (24.88%), fresh weight
(64.77%) and dry weight (61.25%) of cucumber plant, when compared to untreated plants. The
pervious treatment also increased photosynthesis rate, stomatal conductance, and intercellular
CO2 concentration by 50.65%, 15.30% and 12.18%; respectively, compared to the control
treatment. Similar findings were observed in nutrient concentration, carbohydrate content,
Proline, total antioxidants in the leaf, and nutrients. In contrast, eCO2 at 700 ppm in the soil
reduced the values of transpiration rate (6.33%) and Abscisic acid (ABA, 34.03%) content in
cucumber leaves compared to untreated plants under both water levels. Furthermore, the results
revealed that the gene transcript levels of the aquaporin-related genes (CsPIP1-2 and CsTIP4)
significantly increased compared with a well-watered condition. The transcript levels of CsPIP
improved the contribution rate of cell water transportation (intermediated by aquaporin's genes)
and root or leaf hydraulic conductivity. The quantitative real-time PCR expression results
revealed the upregulation of CsAGO1 stress-response genes in plants exposed to 700 ppm CO2.
In conclusion, elevating CO2 at 700 ppm in the soil might be a promising technique to enhance
the growth and productivity of cucumber plants in addition to alleviating the adverse effects of
drought stresses. أجريت تجربتان في صوب متحكم بالظروف بالمناخية الداخلية خلال فصل الشتاء لعامي 2022 و2023. لدراسة تأثير حقن التربة بتركيزات مختلفة لثاني أكسيد الكربون (0، 700 و1000) تحت مستويات ري مختلفة [0، 75% و 100 % بخر نتح للمحصول (ETc) ]. أظهرت النتائج التي تم الحصول عليها أن زيادة تركيز ثاني أكسيد الكربون إلى 700 او 1000 جزء في المليون إلى زيادة معنوية في قياسات نمو النباتات، قياسات التمثيل الضوئي، ومستوى الهرمونات النباتية وكمية محصول للخيار في مستوى الري 75% و100% بخر نتح للمحصول مقارنة بمعاملة الكنترول (CT) في كلا الموسمين.
Text in English and abstract in Arabic & English.
Vegetable
الخضر
Water shortage plant performance elevated CO2 levels gene regulation physiological responses Cucumis sativus نقص المياه أداء نمو النباتات
635
The effect of elevated concentration of soil carbon dioxide with partial root zone drying on the growth rate, yield, water productivity, and fruit quality of cucumber under greenhouse conditions / تأثير زيادة تركيز ثاني أكسيد الكربون في التربة مع التجفيف الجزئي لمنطقة الجذر على معدل النمو، المحصول، إنتاجية المياه، وجودة ثمار الخيار تحت ظروف مناخ الصوبات الزراعية by Mohamed Sayed Mohamed Nageh Ali ; Supervisors Dr. Hassan Ali Hassan, Dr. Emad Abdelhameed Abdeldaym, Dr. Mohamed EL-Sayed Abuarab. - 130 pages : illustrations ; 25 cm. + CD.
Thesis (M.Sc)-Cairo University, 2025.
Bibliography: pages 110-130.
Water scarcity and soil carbon dioxide elevation in arid regions are considered the most serious
factors affecting crop growth and productivity. This study aimed to investigate the impacts of
elevated CO2 levels (eCO2 at rates of 700 and 1000 ppm) on agro-physiological attributes to
induce drought tolerance in cucumbers by activating the expression of genes related to
aquaporin and stress response, which improved the yield of cucumber under two levels of
irrigation water conditions [75% and 100% crop evapotranspiration (ETc)]. Therefore, two field
experiments were conducted in a greenhouse with controlled internal climate conditions, at the
Mohamed Naguib sector of the national company for protected agriculture, during the winter
seasons of 2021-2022 and 2022-2023. The treatments included eCO2 in soil under normal and
partial root zoon drying (PRD, 100% ETc Full irrigations, and 75% ETc). All the applied
treatments were organized as a randomized complete block design (RCBD) and each treatment
was replicated six times. Untreated plants were designed as control treatment (CO2
concentration was 400 ppm). The results of this study showed: that elevating CO2 at 700 and
1000 ppm in soil significantly increased plant growth parameters, photosynthesis
measurements, and phytohormones [indole acetic acid (IAA) and gibberellic acid (GA3)], under
partial root-zone drying (75% ETc) and full irrigation conditions (100% ETc). Under PRD
condition, eCO2 at 700 ppm significantly improved plant height (13.68%), number of shoots
(19.88%), Leaf greenness index (SPAD value, 16.60%), root length (24.88%), fresh weight
(64.77%) and dry weight (61.25%) of cucumber plant, when compared to untreated plants. The
pervious treatment also increased photosynthesis rate, stomatal conductance, and intercellular
CO2 concentration by 50.65%, 15.30% and 12.18%; respectively, compared to the control
treatment. Similar findings were observed in nutrient concentration, carbohydrate content,
Proline, total antioxidants in the leaf, and nutrients. In contrast, eCO2 at 700 ppm in the soil
reduced the values of transpiration rate (6.33%) and Abscisic acid (ABA, 34.03%) content in
cucumber leaves compared to untreated plants under both water levels. Furthermore, the results
revealed that the gene transcript levels of the aquaporin-related genes (CsPIP1-2 and CsTIP4)
significantly increased compared with a well-watered condition. The transcript levels of CsPIP
improved the contribution rate of cell water transportation (intermediated by aquaporin's genes)
and root or leaf hydraulic conductivity. The quantitative real-time PCR expression results
revealed the upregulation of CsAGO1 stress-response genes in plants exposed to 700 ppm CO2.
In conclusion, elevating CO2 at 700 ppm in the soil might be a promising technique to enhance
the growth and productivity of cucumber plants in addition to alleviating the adverse effects of
drought stresses. أجريت تجربتان في صوب متحكم بالظروف بالمناخية الداخلية خلال فصل الشتاء لعامي 2022 و2023. لدراسة تأثير حقن التربة بتركيزات مختلفة لثاني أكسيد الكربون (0، 700 و1000) تحت مستويات ري مختلفة [0، 75% و 100 % بخر نتح للمحصول (ETc) ]. أظهرت النتائج التي تم الحصول عليها أن زيادة تركيز ثاني أكسيد الكربون إلى 700 او 1000 جزء في المليون إلى زيادة معنوية في قياسات نمو النباتات، قياسات التمثيل الضوئي، ومستوى الهرمونات النباتية وكمية محصول للخيار في مستوى الري 75% و100% بخر نتح للمحصول مقارنة بمعاملة الكنترول (CT) في كلا الموسمين.
Text in English and abstract in Arabic & English.
Vegetable
الخضر
Water shortage plant performance elevated CO2 levels gene regulation physiological responses Cucumis sativus نقص المياه أداء نمو النباتات
635