Conservation of endangered medicinal plants natural resources through biotechnology techniques /
Nora Rabee Gowda Abo El-kassem,
Conservation of endangered medicinal plants natural resources through biotechnology techniques / حفظ الموارد الطبيعية للنباتات الطبية المعرضة للانقراض من خلال تقنيات التكنولوجيا الحيوية by Nora Rabee Gowda Abo El-kassem ; Supervisors Dr. Sayed Abdel Kader Fayed, Dr. Mohamed AbdeL-Shakur, Dr. Mai Abdel - Moez Allam. - 110 pages : illustrations ; 25 cm. + CD.
Thesis (Ph.D)-Cairo University, 2025.
Bibliography: pages 89-110.
The study investigated the effects of fourth different shoot induction
media on growth. The first medium was BAv medium, a combination of
0.5 mg L− 1 benzyl adenine (BA), 5 mg L− 1 thiamine HCl, 0.5 mg L− 1
nicotinic acid, and 0.5 mg L− 1 pyridoxine was added to the initial medium.
The second medium was MD medium supplemented with 3 mg L− 1 BA,
0.2 mg L− 1 naphthaleneacetic acid (NAA), 10 mg L− 1 thiamine HCl, 1 mg
L− 1 nicotinic acid, and 1 mg L− 1 pyridoxine. The third medium was ABv
medium formulated with 1 mg L− 1 Adenine sulfate, 1 mg L− 1 benzyl
adenine, 10 mg L− 1 thiamine HCl, 1 mg L− 1 nicotinic acid, and 1 mg L− 1
pyridoxine. The fourth medium AS1v contains 4.4 g L-¹ MS, 30 g L-¹
sucrose, 1 mg L-¹ adenine sulfate, 5 mg L-¹ thiamine HCl, 0.5 mg L-¹
nicotinic acid, 0.5 mg L-¹ pyridoxine, and 2.8 g L-¹ gelrite. and three
media for rooting, the first medium was IB medium supplemented with 0.4
mg L− 1 indol butyric acid, 5 mg L− 1 thiamine HCl, 0.5 mg L− 1 nicotinic
acid, and 0.5 mg L− 1 pyridoxine. The second medium was IA medium
containing 5 mg L− 1 thiamine HCl, 0.5 mg L− 1 nicotinic acid, and 0.5 mg
L− 1 pyridoxine. The third medium was NA media formulated with 0.4 mg
L− 1 naphthaleneacetic acid, 5 mg L− 1 thiamine HCl, 0.5 mg L− 1 nicotinic
acid, and 0.5 mg L− 1 pyridoxine. All media were prepared using Murashige
and Skoog (MS) basal salts 4.4 g L− 1 supplemented with 30 g L− 1 sucrose,
and solidified with 2.8 g L− 1 gelrite. Various propagation protocols were
tested based on the availability of plant materials. HPLC analysis revealed
that the ethanolic extract had the highest quantities of rutin (19.07 µg/mL)
and vanillin (6.29 µg/mL), while the aqueous extract had the highest levels
of gallic acid (7.02 µg/mL) and chlorogenic acid (6.02 µg/mL). SDS-PAGE
examination revealed five protein bands ranging in molecular weight from
28 kDa to 240 kDa. DNA barcoding was used for molecular authentication,
and the acquired sequence was matched to GenBank database sequences
using the BLAST tool. هدفت الدراسة إلى تقييم تأثير ثلاثة أوساط غذائية مختلفة على استحثاث الأفرع .وقد جرى اختبار عدة بروتوكولات إكثار وفقًا لتوافر المواد النباتية.أظهر تحليل HPLC أن المستخلص الإيثانولي احتوى على أعلى تركيز من الروتين (19.07 ميكروجرام/مل) والفانيلين (6.29 ميكروجرام/مل)، بينما سجل المستخلص المائي أعلى نسب من حمض الجاليك (7.02 ميكروجرام/مل) وحمض الكلوروجينيك (6.02 ميكروجرام/مل). كما أوضحت نتائج SDS-PAGE وجود خمسة نطاقات بروتينية تراوحت أوزانها الجزيئية بين 240 إلى 28 كيلودالتون. ولغرض التوثيق الجزيئي تم استخدام DNA barcoding، حيث تمت مقارنة التسلسل الناتج مع قاعدة بيانات GenBank باستخدام أداة BLAST
Text in English and abstract in Arabic & English.
Biotechnology Techniques
تقنيات التكنولوجيا الحيوية
Medicinal plants Endangered plants DNA barcoding SDS HPLC
660.6
Conservation of endangered medicinal plants natural resources through biotechnology techniques / حفظ الموارد الطبيعية للنباتات الطبية المعرضة للانقراض من خلال تقنيات التكنولوجيا الحيوية by Nora Rabee Gowda Abo El-kassem ; Supervisors Dr. Sayed Abdel Kader Fayed, Dr. Mohamed AbdeL-Shakur, Dr. Mai Abdel - Moez Allam. - 110 pages : illustrations ; 25 cm. + CD.
Thesis (Ph.D)-Cairo University, 2025.
Bibliography: pages 89-110.
The study investigated the effects of fourth different shoot induction
media on growth. The first medium was BAv medium, a combination of
0.5 mg L− 1 benzyl adenine (BA), 5 mg L− 1 thiamine HCl, 0.5 mg L− 1
nicotinic acid, and 0.5 mg L− 1 pyridoxine was added to the initial medium.
The second medium was MD medium supplemented with 3 mg L− 1 BA,
0.2 mg L− 1 naphthaleneacetic acid (NAA), 10 mg L− 1 thiamine HCl, 1 mg
L− 1 nicotinic acid, and 1 mg L− 1 pyridoxine. The third medium was ABv
medium formulated with 1 mg L− 1 Adenine sulfate, 1 mg L− 1 benzyl
adenine, 10 mg L− 1 thiamine HCl, 1 mg L− 1 nicotinic acid, and 1 mg L− 1
pyridoxine. The fourth medium AS1v contains 4.4 g L-¹ MS, 30 g L-¹
sucrose, 1 mg L-¹ adenine sulfate, 5 mg L-¹ thiamine HCl, 0.5 mg L-¹
nicotinic acid, 0.5 mg L-¹ pyridoxine, and 2.8 g L-¹ gelrite. and three
media for rooting, the first medium was IB medium supplemented with 0.4
mg L− 1 indol butyric acid, 5 mg L− 1 thiamine HCl, 0.5 mg L− 1 nicotinic
acid, and 0.5 mg L− 1 pyridoxine. The second medium was IA medium
containing 5 mg L− 1 thiamine HCl, 0.5 mg L− 1 nicotinic acid, and 0.5 mg
L− 1 pyridoxine. The third medium was NA media formulated with 0.4 mg
L− 1 naphthaleneacetic acid, 5 mg L− 1 thiamine HCl, 0.5 mg L− 1 nicotinic
acid, and 0.5 mg L− 1 pyridoxine. All media were prepared using Murashige
and Skoog (MS) basal salts 4.4 g L− 1 supplemented with 30 g L− 1 sucrose,
and solidified with 2.8 g L− 1 gelrite. Various propagation protocols were
tested based on the availability of plant materials. HPLC analysis revealed
that the ethanolic extract had the highest quantities of rutin (19.07 µg/mL)
and vanillin (6.29 µg/mL), while the aqueous extract had the highest levels
of gallic acid (7.02 µg/mL) and chlorogenic acid (6.02 µg/mL). SDS-PAGE
examination revealed five protein bands ranging in molecular weight from
28 kDa to 240 kDa. DNA barcoding was used for molecular authentication,
and the acquired sequence was matched to GenBank database sequences
using the BLAST tool. هدفت الدراسة إلى تقييم تأثير ثلاثة أوساط غذائية مختلفة على استحثاث الأفرع .وقد جرى اختبار عدة بروتوكولات إكثار وفقًا لتوافر المواد النباتية.أظهر تحليل HPLC أن المستخلص الإيثانولي احتوى على أعلى تركيز من الروتين (19.07 ميكروجرام/مل) والفانيلين (6.29 ميكروجرام/مل)، بينما سجل المستخلص المائي أعلى نسب من حمض الجاليك (7.02 ميكروجرام/مل) وحمض الكلوروجينيك (6.02 ميكروجرام/مل). كما أوضحت نتائج SDS-PAGE وجود خمسة نطاقات بروتينية تراوحت أوزانها الجزيئية بين 240 إلى 28 كيلودالتون. ولغرض التوثيق الجزيئي تم استخدام DNA barcoding، حيث تمت مقارنة التسلسل الناتج مع قاعدة بيانات GenBank باستخدام أداة BLAST
Text in English and abstract in Arabic & English.
Biotechnology Techniques
تقنيات التكنولوجيا الحيوية
Medicinal plants Endangered plants DNA barcoding SDS HPLC
660.6