Study of the immunopotentiation effect of Esat-6 of mycobacteria on DNA vaccine encoding the H5 gene of avian influenza virus /
Eman Mohamed Samy Ahmed Elnaggar
Study of the immunopotentiation effect of Esat-6 of mycobacteria on DNA vaccine encoding the H5 gene of avian influenza virus / لميكروب السل على لقاح الحامض النووى المشفر لجين H5 لفيروس انفلونزا الطيور Esat-6 دراسة على التأثير المناعى المحفز Eman Mohamed Samy Ahmed Elnaggar ; Supervised Salah Eldien Abdelkarim Selim , Mohamed Ali Mohamed Elhady , Fakreya Abdelhafez Albordeny - Cairo : Eman Mohamed Samy Ahmed Elnaggar , 2014 - 281 P. : charts , facsimiles ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology
In the current study , the DNA vaccine coding for H5 gene of HPA1 H%N1 subtype was produced and co-administrated with DNA vaccine coding for esat6 gene of Mycobacteria (pcDNA3.1/esat6) as an immunomodulatore. first, samples taken from different governorates during 2010 and 2011 were subjected to extensive virus isolation and identification one of 2011 were subjected to extensive virus isolation and identification one of the isolated feed isolate was subjected for further investigation where the full length H5 gene was amplifed and sequenced and cloned in pENTER/D/SD/Topo entry clone and homologues recombination with pDEST40 mammalian expression destination vector was done to produce the DNA vaccine pDEST40/H5
DNA vaccine encoding H5 gene Immunopotentiation
Study of the immunopotentiation effect of Esat-6 of mycobacteria on DNA vaccine encoding the H5 gene of avian influenza virus / لميكروب السل على لقاح الحامض النووى المشفر لجين H5 لفيروس انفلونزا الطيور Esat-6 دراسة على التأثير المناعى المحفز Eman Mohamed Samy Ahmed Elnaggar ; Supervised Salah Eldien Abdelkarim Selim , Mohamed Ali Mohamed Elhady , Fakreya Abdelhafez Albordeny - Cairo : Eman Mohamed Samy Ahmed Elnaggar , 2014 - 281 P. : charts , facsimiles ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology
In the current study , the DNA vaccine coding for H5 gene of HPA1 H%N1 subtype was produced and co-administrated with DNA vaccine coding for esat6 gene of Mycobacteria (pcDNA3.1/esat6) as an immunomodulatore. first, samples taken from different governorates during 2010 and 2011 were subjected to extensive virus isolation and identification one of 2011 were subjected to extensive virus isolation and identification one of the isolated feed isolate was subjected for further investigation where the full length H5 gene was amplifed and sequenced and cloned in pENTER/D/SD/Topo entry clone and homologues recombination with pDEST40 mammalian expression destination vector was done to produce the DNA vaccine pDEST40/H5
DNA vaccine encoding H5 gene Immunopotentiation