Knockout of gene expression in induced endometriotic cells /
Ehab Nabil Salama
Knockout of gene expression in induced endometriotic cells / ايقاف التعبير الجينى فى الخلايا المحفزه الى خلايا مشابهة لخلايا البطانه الرحمية Ehab Nabil Salama ; Supervised Mohamed Amr Hussein Elnoury , Sherif Mohamed Khatab , Osama Mahmoud Azmy - Cairo : Ehab Nabil Salama , 2018 - 164 P. : charts , facsimiles ; 25cm
Thesis (Ph.D.) - Cairo University - National Institute of Laser Enhanced Sciences - Department of Laser Applications in Medical and Biological
Endometriosis is a common gynecological disorder defined as the presence of chronic ectopic functioning endometrial tissues, outside uterine cavity, primarily on the pelvic peritoneum and the ovaries. Several studies showed a correlation between aberrant stem-cell activity in the endometrium and endometriosis. Yet the molecular and cellular behaviors of mesenchymal stem cells in development of endometriosis are hampered by lack of in-vitro experiments. Our aim was to study the morphological and molecular changes associated with mesenchymal stem cells (MSCs) upon exposure to serum derived from women with severe endometriosis. Two cell cultures of MSCs isolated from endometrial tissues of two endometriosis-free women. Each cell culture was treated individually with two concentrations of the serum of women with endometriosis (experimental group/n = 20), and serum of women without endometriosis (control group/ n =10) for 14 days. Quantitative Real-Time PCR was done later to detect expression of OCT4, CDH1 and CDH2, STAT3 and SOX2 genes
Endometriosis Mesenchymal stem cells OCT-4
Knockout of gene expression in induced endometriotic cells / ايقاف التعبير الجينى فى الخلايا المحفزه الى خلايا مشابهة لخلايا البطانه الرحمية Ehab Nabil Salama ; Supervised Mohamed Amr Hussein Elnoury , Sherif Mohamed Khatab , Osama Mahmoud Azmy - Cairo : Ehab Nabil Salama , 2018 - 164 P. : charts , facsimiles ; 25cm
Thesis (Ph.D.) - Cairo University - National Institute of Laser Enhanced Sciences - Department of Laser Applications in Medical and Biological
Endometriosis is a common gynecological disorder defined as the presence of chronic ectopic functioning endometrial tissues, outside uterine cavity, primarily on the pelvic peritoneum and the ovaries. Several studies showed a correlation between aberrant stem-cell activity in the endometrium and endometriosis. Yet the molecular and cellular behaviors of mesenchymal stem cells in development of endometriosis are hampered by lack of in-vitro experiments. Our aim was to study the morphological and molecular changes associated with mesenchymal stem cells (MSCs) upon exposure to serum derived from women with severe endometriosis. Two cell cultures of MSCs isolated from endometrial tissues of two endometriosis-free women. Each cell culture was treated individually with two concentrations of the serum of women with endometriosis (experimental group/n = 20), and serum of women without endometriosis (control group/ n =10) for 14 days. Quantitative Real-Time PCR was done later to detect expression of OCT4, CDH1 and CDH2, STAT3 and SOX2 genes
Endometriosis Mesenchymal stem cells OCT-4