Modern diagnostic techniques to investigate some exotic diseases from Africa /
Sahar Hussien Abdalla Hekal
Modern diagnostic techniques to investigate some exotic diseases from Africa / الطرق الحديثة لاستكشاف بعض الأمراض الوافدة من الدول الأفريقية Sahar Hussien Abdalla Hekal ; Supervised Hassan Mohamed Sobhy , Adelabdel Azim Fayed , Magdy Mahmoud Elsayed - Cairo : Sahar Hussien Abdalla Hekal , 2019 - 181 P. : charts , maps ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of African Postgraduated Studies - Department of Natural Resources
Although animal trade has a valuable role in the economy, it is also associated with the spread of infectious diseases all over the world; the so-called transboundary animal diseases. Therefore the objectives of this study were to determine the seroprevalence of some of these diseases, assess the effectiveness of quarantine measures in the detection of exotic disease and to clarify the role of live animal trade in the spread of infectious transboundary diseases. The study was conducted using 176 serum samples obtained from cattle imported from Sudan to determine the prevalence of Foot and Mouth disease (FMD), Peste Des Petits Ruminants (PPR) and Infectious Bovine Rhinotracheitis (IBR). Three serological tests were performed; Serum Neutralization Test (SNT) for FMD, Indirect enzyme- linked immunosorbent assay (i-ELISA) for PPR and Competitive enzyme- linked immunosorbent assay (cELISA) for IBR.The molecular detection of viral genome of PPRV and BVDV was conducted by real time RT- PCR on DBS and tissue samples. Overall seroprevalence of FMD in tested sera were; 77.27% in the serotype A (A-Iran),68.18% in theserotype A (A- Africa), 93.82% in the serotype O (O- Pan Asia), 59.66% in serotype O (O-Sudan) and 35.227 % in the serotype SAT-2.The overall seroprevalence of PPR was 49.431% while that for IBR was 93.75 %. In the molecular studies there is no viral genome detected in tested samples
Animal trade BVD FMD
Modern diagnostic techniques to investigate some exotic diseases from Africa / الطرق الحديثة لاستكشاف بعض الأمراض الوافدة من الدول الأفريقية Sahar Hussien Abdalla Hekal ; Supervised Hassan Mohamed Sobhy , Adelabdel Azim Fayed , Magdy Mahmoud Elsayed - Cairo : Sahar Hussien Abdalla Hekal , 2019 - 181 P. : charts , maps ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of African Postgraduated Studies - Department of Natural Resources
Although animal trade has a valuable role in the economy, it is also associated with the spread of infectious diseases all over the world; the so-called transboundary animal diseases. Therefore the objectives of this study were to determine the seroprevalence of some of these diseases, assess the effectiveness of quarantine measures in the detection of exotic disease and to clarify the role of live animal trade in the spread of infectious transboundary diseases. The study was conducted using 176 serum samples obtained from cattle imported from Sudan to determine the prevalence of Foot and Mouth disease (FMD), Peste Des Petits Ruminants (PPR) and Infectious Bovine Rhinotracheitis (IBR). Three serological tests were performed; Serum Neutralization Test (SNT) for FMD, Indirect enzyme- linked immunosorbent assay (i-ELISA) for PPR and Competitive enzyme- linked immunosorbent assay (cELISA) for IBR.The molecular detection of viral genome of PPRV and BVDV was conducted by real time RT- PCR on DBS and tissue samples. Overall seroprevalence of FMD in tested sera were; 77.27% in the serotype A (A-Iran),68.18% in theserotype A (A- Africa), 93.82% in the serotype O (O- Pan Asia), 59.66% in serotype O (O-Sudan) and 35.227 % in the serotype SAT-2.The overall seroprevalence of PPR was 49.431% while that for IBR was 93.75 %. In the molecular studies there is no viral genome detected in tested samples
Animal trade BVD FMD