Comparative evaluation of camel and sheep wharton jelly mscs therapy in induced chronic kidney disease model in dogs / (رقم التسجيلة. 163723)
[ عرض عادي ]
| 000 -LEADER | |
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| fixed length control field | 07664nam a2200433Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - أخر تعامل مع التسجيلة | |
| control field | 20260615104136.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 2310s2022 ua a frm 000 0 und d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloguing agency | EG-EULC |
| Language of cataloging | eng |
| Transcribing agency | EG-EULC |
| Modifying agency | EG-EULC |
| Description conventions | rda |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | eng |
| -- | ara |
| 049 ## - Acquisition Source | |
| Acquisition Source | Deposit |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 636.089 |
| 092 ## - LOCALLY ASSIGNED DEWEY CALL NUMBER (OCLC) | |
| Classification number | 636.089 |
| Edition number | 21 |
| 097 ## - Degree | |
| Degree | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Local Call Number | Cai01.10.05.Ph.D.2022.Ta.C. |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Authority record control number or standard number | Tahany Ahmed Ismail Alkolfat, |
| Preparation | preparation. |
| 245 ## - TITLE STATEMENT | |
| Title | Comparative evaluation of camel and sheep wharton jelly mscs therapy in induced chronic kidney disease model in dogs / |
| Statement of responsibility, etc. | by Tahany A.I. Alkolfat ; Supervised Prof.Dr.Hala Mohamed Farouk El Miniawy، Prof.Dr.Haithem Ali Mohamed Farghali, Prof.Dr.ina Sabry Abd El Fattah Essam Mohamed Ibraheem. |
| 246 ## - VARYING FORM OF TITLE | |
| Title proper/short title | تقييم مقارن للعلاج بالخلايا الجذعية الميزينشيمية المستخلصة من الحبل السرى للجمال والأغنام فى نموذج مستحدث لمرض الكلى المزمن فى الكلاب |
| 264 #0 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Date of production, publication, distribution, manufacture, or copyright notice | 2022 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 109 P. : |
| Other physical details | illustrations |
| Dimensions | 25cm+ |
| Accompanying material | Cd |
| 336 ## - CONTENT TYPE | |
| Source | rda content |
| Content type term | text |
| 337 ## - MEDIA TYPE | |
| Source | rdamedia |
| Media type term | Unmediated |
| 338 ## - CARRIER TYPE | |
| Source | rdacarrier |
| Carrier type term | volume |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | .Thesis (Ph.D)-Cairo Univsersity,2022. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Bibliography: p. 96-109. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | chronic kidney disease (CKD) is a critical issue worldwide with an increasing incidence. Treatments available for CKD include dialysis and renal transplantation which have several drawbacks. Xenogeneic cell therapy provides a promising new option in the field of regenerative medicine by using cells from different tissues of animals as source for treating human diseases. Remarkable pre-clinical research needed for the demonstration of efficacy and safety of utilizing xenogeneic cells as therapeutic drug for the progress of this field. This study was carried out to investigate the possible therapeutic effect of both camel and sheep Wharton’s jelly MSC on canine CKD model. The study comprised two parts. Part I was a pilot study to determine the suitable method to induce surgically CKD model in dogs. Part II carried out to investigate the therapeutic effect of both CWJ-MSCs and SWJ-MSCs on the induced CKD model. Part I, the pilot study was applied in three dogs, each one represents one model. Model I, induced by excision of one pole of<br/>right kidney with removal of contralateral kidney after two weeks from first operation. Model II, induced by excision of two poles of right kidney with removal of contralateral kidney after two weeks from the first operation. Model III, was induced by ligation of two renal arterial branches of right kidney, with removal of contralateral kidney after two weeks from the first operation. The results of this study showed that model (2) was considered the best technique for developing a model of canine CKD with persistent uremia.. According to the IRIS staging system in dogs, model (2) represented stage three of CKD with moderate expression of kidney degenerative genes (KIM1& NGAL) and α-SMA in the interstitial tissue and moderate glomerulointerstitial nephritis. The dog in this model became uremic and remained stable for a long time which would allow experimental manipulation. Part II of the study, was performed on nine dogs divided into three groups. chronic kidney disease model induced in dogs by using 5/6 nephrectomy technique. The collected MSCs were isolated and cultured then identified by morphology and flow cytometric analysis. MSCs were injected intrarenal with ultrasonographic guidance in a dose of (5× 106 cells) in two doses. Serum was collected regularly for kidney function test. Kidney tissue specimens were collected for histopathology and identification of NGAL, KIM-1, and EGFR genes by real time PCR. Serum urea and creatinine levels were significantly decreased in the treated groups. The kidney function test recorded more decreased values in the group treated with CWJ-MSCs than that treated with SWJ-MSCs. On the level of histopathology, the lesion scores recorded improvement of glomerular lesions and fibrosis in all experimental cases in the group treated with CWJ-MSCs while in the group treated with SWJ-MSCs the renal lesions improvement varied between experimental cases especially medullary fibrosis. Remarkable thickening of glomerular basement membrane and focal mononuclear cellular reaction were obvious in this group. NGAL, KIM-1genes decreased and EGFR gene increased in all treated groups indicating regeneration.<br/>Conclusion:<br/>SWJ- MSCs transplantation improve renal function tests and enhance tubular regeneration and tissue repair with degree less than CWJ-MSCS but failed to decrease fibrosis.<br/>SWJ- MSCs transplantation in the induced CKD model incite interstitial mononuclear cells infiltration composed mainly of plasma cells and increase thickening of glomerular basement membrane, indicating immunological reaction occurrence. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | • للخلايا الجذعيه الميزنشيمية المستخلصة من نسيج الحبل السري للجمل تأثير علاجي جيد على مرض الكلى المزمن المحدث جراحيا فى الكلاب من خلال تحسين وظائف الكلى وتحسن الصوره الباثولوجيه للمرض بالاضافه الى قدرتها على تقليل التليف. • للخلايا الجذعيه الميزينشيميه المعزوله من الاغنام القدره ايضا على تحسين وظائف الكلى وتحسين الصوره الباثولوجيه لمرض الكلى المزمن فى الكلاب ولكن تأثيرها اقل من نظيرتها المعزوله من الجمل بالاضافه الى عدم قدرتها على تقليل التليف. . • ربما تسببت الخلايا الجذعيه للاغنام فى رد فعل مناعى وذلك من خلال ارتشاح الخلايا الالتهابيه وحيده النواه على الاغلب خلايا البلازما فى النسيج البيبنى للكلى بالاضافه الى وجود سماكه فى الطبقه الجداريه لكبسوله بومان • حقن الخلايا مباشرا فى النسيج الكلوى تسبب فى وجود بعض الرضوض ربما حقن الخلايا قى الشريان الكلوى يكون له نتائج افضل. • واخيرا نحن فى حاجه الى مزيد من الدراسات لمعرفة المواد الالتهابية و انواعها و التى قد تصاحب العلاج بالخلايا الجذعية المنزنشيمة المستخرجة من الحبل السرى بالاضافه الى عمل دراسات مقارنه بين الخلايا الجذعيه الميزنشيميه المعزوله من الحبل السرى لكل من الجمل و الانسان وحيوان اخر وليكن الابقار . . |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Issues CD | Issues also as CD. |
| 546 ## - LANGUAGE NOTE | |
| Text Language | Text in English and abstract in Arabic & English. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Canine kidney disease (CKD) |
| 653 ## - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Sheep Wharton’s jelly mesemchymal stem cells (SWJ- MSCs) |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hala Mohamed Farouk El Miniawy |
| Relator term | thesis advisor. |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Haithem Ali Mohamed Farghali |
| Relator term | thesis advisor. |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Drina Sabry Abd El Fattah Essam Mohamed Ibraheem |
| Relator term | thesis advisor. |
| 900 ## - Thesis Information | |
| Grant date | 01-01-2022 |
| Supervisory body | Hala Mohamed Farouk El Miniawy |
| -- | Haithem Ali Mohamed Farghali |
| -- | Drina Sabry Abd El Fattah Essam Mohamed Ibraheem |
| Universities | Cairo University |
| Faculties | Faculty of veterinary medicine |
| Department | Department of Pathology |
| 905 ## - Cataloger and Reviser Names | |
| Cataloger Name | Shimaa |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Source of classification or shelving scheme | Home library | Current library | Date acquired | Inventory number | Full call number | Barcode | Date last seen | Effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | 85797 | Cai01.10.05.Ph.D.2022.Ta.C. | 01010110085797000 | 30.10.2023 | 30.10.2023 | Thesis |