In vitro assessment of a therapeutic agent effect on human cancer cells / (Record no. 170891)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04943namaa22004331i 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - أخر تعامل مع التسجيلة | |
| control field | 20250325110224.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250218s2024 |||a|||f m||| 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloguing agency | EG-GICUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GICUC |
| Modifying agency | EG-GICUC |
| Description conventions | rda |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | eng |
| -- | ara |
| 049 ## - Acquisition Source | |
| Acquisition Source | Deposit |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 616.994 |
| 092 ## - LOCALLY ASSIGNED DEWEY CALL NUMBER (OCLC) | |
| Classification number | 616.994 |
| Edition number | 21 |
| 097 ## - Degree | |
| Degree | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Local Call Number | Cai01.12.02.M.Sc.2024.La.I. |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Authority record control number or standard number | Laila Osama Mostafa Mohammed, |
| Preparation | preparation. |
| 245 13 - TITLE STATEMENT | |
| Title | In vitro assessment of a therapeutic agent effect on human cancer cells / |
| Statement of responsibility, etc. | by Laila Osama Mostafa Mohammed; supervisors Dr. Heba Mohammed Kamal, Dr. Mohammed Attia Ragheb, Dr. Marwa Hassan Soliman. |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | التقييم المختبري لتأثير عامل علاجي على خلايا سرطانية بشرية / |
| 264 #0 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Date of production, publication, distribution, manufacture, or copyright notice | 2024. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 101 pages : |
| Other physical details | illustrations ; |
| Dimensions | 25 cm. + |
| Accompanying material | CD. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Source | rda content |
| 337 ## - MEDIA TYPE | |
| Media type term | Unmediated |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | volume |
| Source | rdacarrier |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.)-Cairo University, 2024. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Bibliography: pages 84-98. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | A series of novel chalcone compounds (3a-f) have been synthesized and their structures were characterized via different spectral techniques. Their anticancer potentials were studied in vitro via MTT assay against four human cancer cell lines, including MDA-MB231 (breast cancer), A549 (lung cancer), HeLa (cervical cancer cells), and HepG2 (liver cancer cells), and a non-cancerous cell line WI-38(normal lung tissue). The most responsive cell to the synthetic compounds was MDA-MB-231, and the most potent derivative was 3c, which had a methoxy group and a remarkable IC50 value of 21.87 µM. Mitochondrial membrane potential (ΔΨm), dual acridine orange/ethidium bromide (AO/EB) fluorescent staining and scanning electron microscope (SEM) investigations revealed that 3c had a significant role in apoptosis induction in MDA-MB-231 cells. Furthermore, 3c induced a substantial induction of cell cycle arrest of MDA-MB-231 cells at G0-G1 and G2/M phases and significantly suppressed cell migration. Protein-expression analysis using ELISA assay provided valuable insight into the underlying molecular mechanism of 3c antitumor action. Interestingly, 3c could remarkably induce the protein expression of proapoptotic (Bax and Caspase 3) and epithelial (E-cadherin) markers, while the expression of antiapoptotic marker (Bcl2) and cell cycle regulators (Cyclin D and CDK4/6) were downregulated. The DNA binding affinity of 3c was also investigated using UV-Vis spectral analysis Collectively, these results emphasize the potential of 3c as a multifaceted approach to breast cancer treatment, combining potent cytotoxicity, apoptosis induction, and migration inhibition. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | تم تخليق سلسلة من مركبات الشالكون الجديدة وتوصيفها بواسطة تحليل الرنين المغناطيسي النووي .(NMR (تمت دراسة خصائصها المضادة للسرطان ضد أربعة خطوط خاليا سرطانية بشرية مختلفة باستخدام تحليل In MTT .vitroأظهرت الدراسات أن المركبc 3هو األكثر فعالية ضد سرطان الثدي -231MB-MDA بقيمة 50IC تبلغ لحو ًظ .µM21,87 . درست الفحوصات األولية دور المركبc 3 في البرمجة الذاتية للخاليا، حيث أظهر تأثي ًرا م ا ًطا كبي ًرا لهجرة الخاليا. كما أدى المركب إلى توقف دورة الخلية للخاليا-MDA على الغشاء الميتوكندريالي وتثبي -231MBفي مراحل 1G0-G وM2/G، مع تأثيرات واضحة على تعبير البروتينات المتعلقة بالبروتابتوز المحرض للبرمجة الذاتية والطبقة الظاهرية. يظهر تحليل الطيف األشعة فوق البنفسجية المرئية قدرة المركب على ربط الحمض النووي. يشير هذا البحث إلى إمكانية استخدام المركب c 3 كمرشح واعد لعالج سرطان الثدي، مع التأكيد على ضرورة مزيد من الدراسات لتحديد تأثيره في النماذج السريرية المبدئية. |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Issues CD | Issued also as CD |
| 546 ## - LANGUAGE NOTE | |
| Text Language | Text in English and abstract in English. |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | cancer |
| Source of heading or term | qrmak |
| 653 #0 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Chalcone |
| -- | Breast cancer |
| -- | Cell death |
| -- | Migration |
| -- | DNA binding |
| -- | ELISA |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Heba Mohammed Kamal |
| Relator term | thesis advisor. |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohammed Attia Ragheb |
| Relator term | thesis advisor. |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Marwa Hassan Soliman |
| Relator term | thesis advisor. |
| 900 ## - Thesis Information | |
| Grant date | 01-01-2024 |
| Supervisory body | Heba Mohammed Kamal |
| -- | Mohammed Attia Ragheb |
| -- | Marwa Hassan Soliman |
| Universities | Cairo University |
| Faculties | Faculty of Science |
| Department | Department of Chemistry |
| 905 ## - Cataloger and Reviser Names | |
| Cataloger Name | Sara Salah |
| Reviser Names | Eman Ghareb |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Edition | 21 |
| Suppress in OPAC | No |
| Source of classification or shelving scheme | Home library | Current library | Date acquired | Inventory number | Full call number | Barcode | Date last seen | Effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 18.02.2025 | 90628 | Cai01.12.02.M.Sc.2024.La.I. | 01010110090628000 | 18.02.2025 | 18.02.2025 | Thesis |