MARC details
| 000 -LEADER |
|---|
| fixed length control field |
11169namaa22004331i 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
OSt |
| 005 - أخر تعامل مع التسجيلة |
|---|
| control field |
20250923113846.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
250717s2024ua |||a|||frm||| 000 0 eng d |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloguing agency |
EG-GICUC |
| Language of cataloging |
eng |
| Transcribing agency |
EG-GICUC |
| Modifying agency |
EG-GICUC |
| Description conventions |
rda |
| 041 0# - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| Language code of summary or abstract |
eng |
| -- |
ara |
| 049 ## - Acquisition Source |
|---|
| Acquisition Source |
Deposit |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
|---|
| Classification number |
636.089 |
| 092 ## - LOCALLY ASSIGNED DEWEY CALL NUMBER (OCLC) |
|---|
| Classification number |
636.089 |
| Edition number |
21 |
| 097 ## - Degree |
|---|
| Degree |
Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
|---|
| Local Call Number |
Cai01.10.10.Ph.D.2024.Sa.Q. |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Authority record control number or standard number |
Sara Mahmoud Ibrahim Ahmed, |
| Preparation |
preparation. |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Genetic Diversity Analysis of Highly Polymorphic Genes in Methicillin-Resistant Staphylococcus aureus / |
| Statement of responsibility, etc. |
By Sara Mahmoud Ibrahim Ahmed; Under Supervision of Prof. Dr. Jakeen Kamal Abd Elhalem El-Jakee, Prof. Dr. Mahmoud Dardiri El Hariri, Prof. Dr. Haithem Ali Farghali. |
| 246 15 - VARYING FORM OF TITLE |
|---|
| Title proper/short title |
ﺗﺣﻠﯾل اﻟﺗﻧوع اﻟوراﺛﻲ ﻟﻠﺟﯾﻧﺎت ﻋﺎﻟﯾﺔ اﻟﺗﻌدد اﻟﺷﻛﻠﻲ ﻓﻲ ﺳﺗﺎﻓﯾﻠوﻛوﻛس اورﯾس اﻟﻣﻘﺎوﻣﺔ ﻟﻠﻣﺳﯾﺳﻠﯾن |
| 264 #0 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
|---|
| Date of production, publication, distribution, manufacture, or copyright notice |
2024. |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
86 pages : |
| Other physical details |
illustrations ; |
| Dimensions |
25 cm. + |
| Accompanying material |
CD. |
| 336 ## - CONTENT TYPE |
|---|
| Content type term |
text |
| Source |
rda content |
| 337 ## - MEDIA TYPE |
|---|
| Media type term |
Unmediated |
| Source |
rdamedia |
| 338 ## - CARRIER TYPE |
|---|
| Carrier type term |
volume |
| Source |
rdacarrier |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Thesis (Ph.D)-Cairo University, 2024. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
|---|
| Bibliography, etc. note |
Bibliography: pages 70-86. |
| 520 #3 - SUMMARY, ETC. |
|---|
| Summary, etc. |
Methicillin-resistant Staphylococcus aureus (MRSA) poses a significant public health threat globally, impacting both human and Veterinary Medicine. This study aimed to comprehensively investigate the prevalence, antibiotic susceptibility, and molecular characteristics of MRSA isolates from humans, dogs, and cats in Egypt. A total of 561 swab samples were meticulously collected from clinical cases and apparently healthy animals, with an additional 30 MRSA isolates obtained from humans. Extensive phenotypic identification and antibiotic susceptibility testing uncovered alarming resistance rates among MRSA isolates, particularly notable against oxacillin, cefoxitin, penicillin, and norfloxacin. Notably, all MRSA isolates unequivocally harbored the mecA gene, indicating robust methicillin resistance. Furthermore, molecular analysis through PCR assays targeting mecA, mecC, spa, and nuc genes provided intricate insights into the genetic landscape of the isolates. Intriguingly, a subset of MRSA isolates exhibited partial deletion of the nuc gene, posing significant diagnostic challenges. Phylogenetic analysis unveiled a strikingly high genetic similarity among MRSA isolates from diverse species origins, hinting at potential interspecies transmission dynamics. Importantly, detailed examination of the MRSA isolates' origins revealed intriguing patterns: canine samples exhibited a prevalence of approximately 5.5%, with 13 positive MRSA cases, while feline samples yielded 8 MRSA isolates, resulting in a prevalence of 2.5%. Unfortunately, due to sample unavailability, the incidence among humans was not estimated in this study. These findings underscore the pivotal role of pets, particularly dogs and cats, as potential reservoirs for MRSA transmission within communities. This study focuses on the urgent need for enhanced surveillance and robust control measures to curtail the spread of MRSA, considering both human and animal health ramifications.This study investigates also the efficacy of Platelet-Rich Plasma (PRP) alone and in combination with ciprofloxacin ear drops in treating Methicillin-resistant Staphylococcus aureus (MRSA) infected otitis externa in dogs. Seven dogs with bilateral MRSA otitis externa were enrolled, receiving ciprofloxacin ear drops in one ear and PRP drops in the other, either alone or combined. Clinical, cytological, and microbiological evaluations were conducted at four time points over 15 days. Results showed that the combined treatment significantly reduced MRSA load, with higher clinical and cytological recovery rates compared to single treatments. PRP, known for its regenerative properties, enhanced the antimicrobial activity of ciprofloxacin, suggesting a synergistic effect. This combined approach holds promise for effectively treating MRSA-infected otitis externa in dogs, offering a potentially safer and more sustainable alternative to traditional antibiotic therapy.<br/> |
| 520 #3 - SUMMARY, ETC. |
|---|
| Summary, etc. |
ﻣﯿﻜﺮوﺑﺎت اﻻﺳﺘﺎﻓﯿﺎوﻛﻮﻛﺲ اورﯾﺲ اﻟﻤﻘﺎوﻣﺔ ﻟﻠﻤﯿﺜﯿﺴﯿﻠﯿﻦ (MRSA) ﺗﺸﻜﻞ ﺗﮭﺪﯾﺪًا ﻛﺒﯿ ًﺮا ﻟﻠﺼﺤﺔ اﻟﻌﺎﻣﺔ ﻋﻠﻰ اﻟﺼﻌﯿﺪﯾﻦ اﻟﻌﺎﻟﻤﻲ واﻟﻤﺤﻠﻲ، ﻣﻤﺎ ﯾﺆﺛﺮ ﻋﻠﻰ اﻟﻄﺐ اﻟﺒﺸﺮي واﻟﺒﯿﻄﺮي ﻋﻠﻰ ﺣﺪ ﺳﻮاء. ھﺬه اﻟﺪراﺳﺔ ﺗﮭﺪف إﻟﻰ اﺳﺘﻜﺸﺎف اﻧﺘﺸﺎر ﻣﯿﻜﺮوﺑﺎت اﻻﺳﺘﺎﻓﯿﺎوﻛﻮﻛﺲ اورﯾﺲ اﻟﻤﻘﺎوﻣﺔ ﻟﻠﻤﯿﺜﯿﺴﯿﻠﯿﻦ (MRSA)، ودرﺟﺔ ﺣﺴﺎﺳﯿﺘﮭﺎ ﻟﻠﻤﻀﺎدات اﻟﺤﯿﻮﯾﺔ، واﻟﺴﻤﺎت اﻟﺠﺰﯾﺌﯿﺔ ﻟﮭﺬة اﻟﻌﺘﺮات اﻟﻤﻌﺰوﻟﺔ ﻣﻦ اﻟﺒﺸﺮ واﻟﻜﻼب واﻟﻘﻄﻂ ﻓﻲ ﻣﺼﺮ. ﺗﻢ ﺗﺠﻤﯿﻊ ﻋﺪد 561 ﻋﯿﻨﺔ ﻣﻦ اﻟﻘﻄﻂ واﻟﻜﻼب، ﺑﺎﻹﺿﺎﻓﺔ إﻟﻰ 30 ﻣﻌﺰوﻟﺔ اﺳﺘﺎﻓﯿﺎوﻛﻮﻛﺲ اورﯾﺲ ﻣﻘﺎوﻣﺔ ﻟﻠﻤﯿﺜﯿﺴﯿﻠﯿﻦ (MRSA) ﻣﻦ اﻟﺒﺸﺮ. ﻛﺸﻔﺖ اﻟﻔﺤﻮﺻﺎت اﻟﻈﺎھﺮﯾﺔ واﺧﺘﺒﺎرات ﺣﺴﺎﺳﯿﺔ اﻟﻤﻀﺎدات اﻟﺤﯿﻮﯾﺔ ﻋﻦ ارﺗﻔﺎع ﻣﻌﺪﻻت اﻟﻤﻘﺎوﻣﺔ ﻟﻠﻤﻀﺎدات اﻟﺤﯿﻮﯾﺔ ﺑﯿﻦ ﻣﻌﺰوﻻت اﻻﺳﺘﺎﻓﯿﺎوﻛﻮﻛﺲ اورﯾﺲ اﻟﻤﻘﺎوﻣﺔ ﻟﻠﻤﯿﺜﯿﺴﯿﻠﯿﻦ (MRSA)، ﺧﺎﺻﺔ ﺿﺪ اﻷوﻛﺴﺎﺳﯿﻠﯿﻦ، واﻟﺴﯿﻔﻮﻛﺴﯿﺘﯿﻦ، واﻟﺒﻨﯿﺴﯿﻠﯿﻦ، واﻟﻨﻮرﻓﻠﻮﻛﺴﺎﺳﯿﻦ. ﺑﺎﻹﺿﺎﻓﺔ إﻟﻰ ذﻟﻚ اﺛﺒﺖ اﺧﺘﺒﺎر ﺗﻔﺎﻋﻞ اﻟﺒﻠﻤﺮة اﻟﻤﺘﺴﻠﺴﻞ ان ﺟﻤﯿﻊ ﻣﻌﺰوﻻت اﻻﺳﺘﺎﻓﯿﺎوﻛﻮﻛﺲ اورﯾﺲ اﻟﻤﻘﺎوﻣﺔ ﻟﻠﻤﯿﺜﯿﺴﯿﻠﯿﻦ (MRSA) ﺗﺤﻤﻞ ﺟﯿﻦ ﻣﻚ أ mecA، ﻣﻤﺎ ﯾﺪل ﻋﻠﻰ ﻣﻘﺎوﻣﺔ ﻗﻮﯾﺔ ﻟﻠﻤﯿﺜﯿﺴﯿﻠﯿﻦ. ﻛﻤﺎ ﻛﺸﻒ اﻟﺘﺤﻠﯿﻞ اﻟﺠﺰﯾﺌﻲ ﻟﻠﻤﻌﺰوﻻت ﻋﻦ ﻣﻌﻠﻮﻣﺎت دﻗﯿﻘﺔ ﺣﻮل اﻟﺴﻤﺎت اﻟﺠﯿﻨﯿﺔ ﻟﻠﻤﻌﺰوﻻت ﻟﻠﻜﺸﻒ ﻋﻦ ﺟﯿﻨﺎت ﻣﺴﺘﮭﺪﻓﺔ mecA، وmecC، وspa، و.nuc وﻣﻦ اﻟﺠﺪﯾﺮ ﺑﺎﻟﺬﻛﺮ أن ﺑﻌﺾ اﻟﻤﻌﺰوﻻت أظﮭﺮت ﺣﺬﻓًﺎ ﺟﺰﺋﯿًﺎ ﻟﺠﯿﻦ ﻧﻚ nuc، ﻣﻤﺎ ﯾﺸﻜﻞ ﺗﺤﺪﯾﺎت ﺗﺸﺨﯿﺼﯿﺔ ﻛﺒﯿﺮة. وﻛﺸﻒ ﺗﺤﻠﯿﻞ اﻟﺘﺘﺎﺑﻊ اﻟﺠﯿﻨﻰ (اﻟﻔﯿﻠﻮﺟﯿﻨﻲ) ﻋﻦ ﺗﺸﺎﺑﮫ ﺟﯿﻨﻲ ﻣﻠﺤﻮظ ﺑﯿﻦ ﻣﻌﺰوﻻت اﻻﺳﺘﺎﻓﯿﺎوﻛﻮﻛﺲ اورﯾﺲ اﻟﻤﻘﺎوﻣﺔ ﻟﻠﻤﯿﺜﯿﺴﯿﻠﯿﻦ (MRSA) ﻣﻦ أﺻﻮل وأﻧﻮاع ﻣﺨﺘﻠﻔﺔ، ﻣﻤﺎ ﯾﺸﯿﺮ إﻟﻰ وﺟﻮد اﻧﺘﻘﺎل ﻣﺤﺘﻤﻞ ﺑﯿﻦ اﻷﻧﻮاع. اطﮭﺮت ﻧﺘﺎﺋﺞ اﻟﺪراﺳﺔ<br/>اﻧﺘﺸﺎرا ﻣﯿﻜﺮوب اﻻﺳﺘﺎﻓﯿﺎوﻛﻮﻛﺲ اورﯾﺲ اﻟﻤﻘﺎوم ﻟﻠﻤﯿﺜﯿﺴﯿﻠﯿﻦ (MRSA) ﺑﯿﻦ اﻟﻜﻼب واﻟﻘﻄﻂ ، ﻣﻤﺎ ﯾﺒﺮز اﻟﺪور اﻟﻤﺤﺘﻤﻞ ﻟﻠﺤﯿﻮاﻧﺎت اﻷﻟﯿﻔﺔ ﻓﻲ اﻧﺘﻘﺎل ھﺬةه اﻟﻤﯿﻜﺮوﺑﺎت داﺧﻞ اﻟﻤﺠﺘﻤﻌﺎت. ﻟﺬا، ﯾﻌﺪ ﺗﻌﺰﯾﺰ اﻟﺮﺻﺪ واﺗﺨﺎذ اﺟﺮاءات ﻣﻜﺎﻓﺤﺔ ﻓﻌﺎﻟﺔ أﻣﺮا ﺿﺮورﯾًﺎ ﻻﺣﺘﻮاء اﻧﺘﺸﺎراﻻﺳﺘﺎﻓﯿﺎوﻛﻮﻛﺲ اورﯾﺲ اﻟﻤﻘﺎوﻣﺔ ﻟﻠﻤﯿﺜﯿﺴﯿﻠﯿﻦ (MRSA) واﻟﺤﺪ ﻣﻦ آﺛﺎره ﻋﻠﻰ ﺻﺤﺔ اﻻﻧﺴﺎن واﻟﺤﯿﻮان.<br/><br/>ھﺪﻓﺖ ھﺬه اﻟﺪراﺳﺔ اﯾﻀﺎ اﻟﻰ ﺗﻘﯿﻢ ﻓﻌﺎﻟﯿﺔ اﻟﺒﻼزﻣﺎ اﻟﻐﻨﯿﺔ ﺑﺎﻟﺼﻔﺎﺋﺢ (PRP) ﺑﻤﻔﺮدھﺎ او ﻓﻲ ﻣﺰﯾﺞ ﻣﻊ ﻗﻄﺮات اﻷذن ﺳﯿﺒﺮوﻓﻠﻮﻛﺴﺎﺳﯿﻦ ﻓﻲ ﻋﻼج اﻟﺘﮭﺎب اﻷذن اﻟﺨﺎرﺟﯿﺔ اﻟﻤﺼﺎﺣﺐ ﻟﻌﺪوى ﺑﻜﺘﯿﺮﯾﺎ اﻻﺳﺘﺎﻓﯿﺎوﻛﻮﻛﺲ اورﯾﺲ اﻟﻤﻘﺎوﻣﺔ ﻟﻠﻤﯿﺜﯿﺴﯿﻠﯿﻦ (MRSA) ﻓﻲ اﻟﻜﻼب. ﺗﻢ اﺟﺮاء ﺗﺠﺮﺑﺔ ﻋﻠﻰ ﻋﺪد ﺳﺒﻊ ﻛﻼب ﻣﺼﺎﺑﺔ ﺑﺎﻟﺘﮭﺎب اﻷذن اﻟﺨﺎرﺟﯿﺔ اﻟﻤﺰدوج اﻟﺠﺎﻧﺐ ﻣﺼﺤﻮب ﺑﻌﺪوى اﻻﺳﺘﺎﻓﯿﺎوﻛﻮﻛﺲ اورﯾﺲ اﻟﻤﻘﺎوﻣﺔ ﻟﻠﻤﯿﺜﯿﺴﯿﻠﯿﻦ (MRSA)، ﺣﯿﺚ ﺗﻠﻘﺖ ﻗﻄﺮات اﻷذن ﺳﯿﺒﺮوﻓﻠﻮﻛﺴﺎﺳﯿﻦ ﻓﻲ أﺣﺪ اﻷذﻧﯿﻦ وﻗﻄﺮات اﻟﺒﻼزﻣﺎ اﻟﻐﻨﯿﺔ ﺑﺎﻟﺼﻔﺎﺋﺢ PRP ﻓﻲ اﻷذن اﻷﺧﺮى، إﻣﺎ ﺑﻤﻔﺮدھﺎ أو ﻣﺠﺘﻤﻌﺔ. ﺗﻢ إﺟﺮاء ﺗﻘﯿﯿﻤﺎت ﺳﺮﯾﺮﯾﺔ وﺧﻠﻮﯾﺔ وﻣﯿﻜﺮوﺑﯿﻮﻟﻮﺟﯿﺔ ﻓﻲ أرﺑﻊ ﻧﻘﺎط زﻣﻨﯿﺔ ﻋﻠﻰ ﻣﺪى 15 ﯾﻮ ًﻣﺎ. اﺛﺒﺘﺖ اﻟﻨﺘﺎﺋﺞ ان اﻟﺒﻼزﻣﺎ اﻟﻐﻨﯿﺔ ﺑﺎﻟﺼﻔﺎﺋﺢ PRP اﻟﻤﻌﺮوﻓﺔ ﺑﺨﺼﺎﺋﺼﮭﺎ اﻟﺘﺠﺪدﯾﺔ، زادت ﻣﻦ اﻟﻨﺸﺎط اﻟﻤﻀﺎد ﻟﻠﻤﯿﻜﺮوﺑﺎت ﻟﺴﯿﺒﺮوﻓﻠﻮﻛﺴﺎﺳﯿﻦ، ﻣﻤﺎ ﯾﻮﺣﻲ ﺑﺘﺄﺛﯿﺮ ﺗﻌﺎوﻧﻲ. وﯾﻌﺪ ھﺬا اﻟﻨﮭﺞ وﺳﯿﻠﺔ ﻓﻌﺎﻟﺔ واﻋﺪة ﻟﻌﻼج ﺑﻜﺘﯿﺮﯾﺎ اﻻﺳﺘﺎﻓﯿﺎوﻛﻮﻛﺲ اورﯾﺲ اﻟﻤﻘﺎوﻣﺔ ﻟﻠﻤﯿﺜﯿﺴﯿﻠﯿﻦ (MRSA) اﻟﻤﺼﺎﺣﺒﺔ ﻻﻟﺘﮭﺎب اﻷذن اﻟﺨﺎرﺟﯿﺔ ﻓﻲ اﻟﻜﻼب، ﻣﻤﺎ ﯾﻘﺪم ﺑﺪﯾﻼً أﻛﺜﺮ أﻣﺎﻧًﺎ واﺳﺘﺪاﻣﺔ ﻋﻦ اﻟﻌﻼج ﺑﺎﻟﻤﻀﺎدات اﻟﺤﯿﻮﯾﺔ اﻟﺘﻘﻠﯿﺪﯾﺔ.<br/> |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
|---|
| Issues CD |
Issued also as CD |
| 546 ## - LANGUAGE NOTE |
|---|
| Text Language |
Text in English and abstract in Arabic & English. |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Veterinary Medicine /Microbiology |
| 653 #1 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
antibiotic resistance |
| -- |
human, canine |
| -- |
Methicillin resistant Staphylococcus aureus (MRSA) |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Jakeen Kamal Abd Elhalem El-Jakee |
| Relator term |
thesis advisor. |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Mahmoud Dardiri El Hariri |
| Relator term |
thesis advisor. |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Haithem Ali Farghali |
| Relator term |
thesis advisor. |
| 900 ## - Thesis Information |
|---|
| Grant date |
01-01-2024 |
| Supervisory body |
Jakeen Kamal Abd Elhalem El-Jakee |
| -- |
Mahmoud Dardiri El Hariri |
| -- |
Haithem Ali Farghali |
| Universities |
Cairo University |
| Faculties |
Faculty of Veterinary Medicine |
| Department |
Department of Microbiology |
| 905 ## - Cataloger and Reviser Names |
|---|
| Cataloger Name |
Eman El gebaly |
| Reviser Names |
Eman Ghareb |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Thesis |
| Edition |
21 |
| Suppress in OPAC |
No |