Molecular and computational investigation of the skin microbiota associated with Pityriasis infection / (Record no. 175210)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 06916namaa22004451i 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GICUC |
| 005 - أخر تعامل مع التسجيلة | |
| control field | 20251026135809.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 251026s2025 ua a|||frm||| 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloguing agency | EG-GICUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GICUC |
| Modifying agency | EG-GICUC |
| Description conventions | rda |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | eng |
| -- | ara |
| 049 ## - Acquisition Source | |
| Acquisition Source | Deposit |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 616.01 |
| 092 ## - LOCALLY ASSIGNED DEWEY CALL NUMBER (OCLC) | |
| Classification number | 616.01 |
| Edition number | 21 |
| 097 ## - Degree | |
| Degree | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Local Call Number | Cai01.08.06.M.Sc.2025.Mo.M |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Authority record control number or standard number | Mohamed Taha Abdelaziz, |
| Preparation | preparation. |
| 245 10 - TITLE STATEMENT | |
| Title | Molecular and computational investigation of the skin microbiota associated with Pityriasis infection / |
| Statement of responsibility, etc. | by Mohamed Taha Abdelaziz ; Supervision Dr. Ramy Karam Aziz, Dr. Mariam Hassan Haikal, Dr. Dalia Ali Eldamacy. |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | دراسات جزيئية وحاسوبية للتجمعات الميكروبية الجلدية المرتبطة بعدوي النخالية المبرقشة |
| 264 #0 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Date of production, publication, distribution, manufacture, or copyright notice | 2025. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 72 pages : |
| Other physical details | illustrations ; |
| Dimensions | 25 cm. + |
| Accompanying material | CD. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Source | rda content |
| 337 ## - MEDIA TYPE | |
| Media type term | Unmediated |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | volume |
| Source | rdacarrier |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc)-Cairo University, 2025. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Bibliography: pages 61-72. |
| 520 #3 - SUMMARY, ETC. | |
| Summary, etc. | Pityriasis versicolor is a superficial fungal infection that causes skin pigmentation lesions, mostly without any severe symptoms but with a high prevalence and incidence of recurrence. An altered skin microbial community with an increase in Malassezia species, predominately Malassezia furfur, together with the transformation of those pityrosporum Malassezia to their mycelial form, are reported as the main cause of the disease. Despite the substantial advances in studying the human skin microbiome over the past two decades, the distribution of M. furfur in different skin microbiomes, and the involvement of the skin bacteriome in pityriasis versicolor are not conclusive. This study was initiated to systematically determine the distribution of M. furfur in sequenced skin metagenomes and to experimentally investigate its association with skin bacteriome profiles, which may provide clues to the role of skin microbiota balance in the treatment of pityriasis versicolor. First, publicly available skin metagenomes were computationally analyzed to systematically determine the distribution of M. furfur and its relative abundance at different skin sites. Using BLASTN to map newly proposed marker genes to representative metagenomic datasets allowed the estimation of M. furfur relative abundance, which indicated a relative enrichment of M. furfur in retro auricular crease, antecubital fossa, and forehead samples. Among skin categories, sebaceous areas were the most significantly enriched in M. furfur, while in terms of exposure/occlusion, exposed areas had the highest relative abundance. Second, an experimental case-control study investigated how the skin bacteriome, its dysbiosis, and fungal colonization might be related to pityriasis versicolor infection. The study was conducted on 20 skin swabs (taken from the neck to the subclavius area) from ten patients with pityriasis versicolor and six swabs from healthy volunteers (healthy controls). For each patient, one swab was taken from the lesion, while the other from a non-lesion area. The bacteriome of the 26 samples was profiled by 16S rRNA amplicon sequencing. Bioinformatics analysis indicated that samples from healthy control were more diverse and significantly differed (p-value < 0.05) in their bacterial composition from patients with pityriasis versicolor samples (lesion and non-lesion samples). Betaproteobacteria and Alphaproteobacteria had lower relative abundance in pityriasis versicolor patient skin, while Gammaproteobacteria was slightly more abundant. On the genus level, Ochrobactrum was relative more abundant in healthy skin samples, while Facklamia was relatively more abundant in lesion and non-lesion samples of patients with pityriasis versicolor. In conclusion, skin fungal alteration associated with pityriasis versicolor correlated with remarkable skin bacterial dysbiosis, not only in skin lesions, but also in the non-lesion areas. This finding suggests that bacterial dysbiosis may correlate with pityriasis versicolor infection and recurrence. Moreover, identifying commonly altered disease-associated bacterial taxa is a first step towards potential intervention to restore normal diversity, thus reducing the risk pityriasis versicolor and improving patient recovery. |
| 520 #3 - SUMMARY, ETC. | |
| Summary, etc. | تحتوي الرسالة على اربعة فصول. الفصل الاول يتضمن مقدمة حول الطرق المختلفة لتشييد مشتقات الثيينو]٢,٣-د[بيريميدين، ومقدمة موجزة عن موت الخلايا المبرمج، وإنزيمات مستقبلات التيروزين كيناز، وموت الخلايا المبرمج، بالإضافة إلى النشاط المضاد للسرطان لمشتقات الثينوبيريميدين. الفصل الثاني يعرض أهداف البحث وعرض المخططات التي توضح الطرق العملية التي صممت للحصول على هذه المركبات.<br/> الفصل الثالث يتضمن المناقشة النظرية للجزء العملى، ومناقشة الأنشطة المضادة للسرطان والأنشطة الإنزيمية للمركبات الجديدة، ودراسة النمذجة الجزيئية، ومناقشة موجزة عن علاقة تركيب المركبات بفاعليتها. الفصل الرابع يشمل الخطوات العملية التي تم تنفيذها لتحضير المركبات الوسيطة والمستهدفة بالتفصيل، بالإضافة إلى خصائص المركبات التي تم تشييدها، وتحليل العناصر والبيانات الطيفية لهذه المركبات. هذا الفصل يغطي أيضا النشاط المضاد للسرطان في المختبر وفاعلية المركبات التي تم تشييدها حديثًا ضد إنزيم FLT-3 ، ونشاط موت الخلايا المبرمج للمركبات الأكثر فاعلية ودراسة النمذجة الجزيئية لها. |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Issues CD | Issues also as CD. |
| 546 ## - LANGUAGE NOTE | |
| Text Language | Text in English and abstract in Arabic & English. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Microbiology |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | الميكروبيولوجيا الطبية |
| 653 #1 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | 16S rRNA gene sequencing |
| -- | BLASTN |
| -- | Dysbiosis |
| -- | Malassezia furfur |
| -- | Metagenomics |
| -- | Pityriasis versicolor |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ramy Karam Aziz |
| Relator term | thesis advisor. |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mariam Hassan Haikal |
| Relator term | thesis advisor. |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dalia Ali Eldamacy |
| Relator term | thesis advisor. |
| 900 ## - Thesis Information | |
| Grant date | 01-01-2025 |
| Supervisory body | Ramy Karam Aziz |
| -- | Mariam Hassan Haikal |
| -- | Dalia Ali Eldamacy |
| Universities | Cairo University |
| Faculties | Faculty of Pharmacy |
| Department | Department of Microbiology and Immunology |
| 905 ## - Cataloger and Reviser Names | |
| Cataloger Name | Shimaa |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Edition | 21 |
| Suppress in OPAC | No |
| Source of classification or shelving scheme | Home library | Current library | Date acquired | Inventory number | Full call number | Barcode | Date last seen | Effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 26.10.2025 | 92263 | Cai01.08.06.M.Sc.2025.Mo.M | 01010110092263000 | 26.10.2025 | 26.10.2025 | Thesis |