Biodiversity of salt- affected microbiota associated to natural halophytic flora / (Record no. 175733)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 06019namaa22004451i 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GICUC |
| 005 - أخر تعامل مع التسجيلة | |
| control field | 20251111102743.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 251110s2025 ua a|||frm||| 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloguing agency | EG-GICUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GICUC |
| Modifying agency | EG-GICUC |
| Description conventions | rda |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | eng |
| -- | ara |
| 049 ## - Acquisition Source | |
| Acquisition Source | Deposit |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 660.62 |
| 092 ## - LOCALLY ASSIGNED DEWEY CALL NUMBER (OCLC) | |
| Classification number | 660.62 |
| Edition number | 21 |
| 097 ## - Degree | |
| Degree | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Local Call Number | Cai01.07.06.M.Sc.2025.Na.B |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Authority record control number or standard number | Nada Ahmed Moner, |
| Preparation | preparation. |
| 245 10 - TITLE STATEMENT | |
| Title | Biodiversity of salt- affected microbiota associated to natural halophytic flora / |
| Statement of responsibility, etc. | by Nada Ahmed Moner ; Supervisors Dr.Nabil Abrahim Hegazi, Dr. Mohamed Fayez Fouad, Dr.Tarek Sayed Ragab. |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | التنوع الحيوي للميكروبات المصاحبة للفلورا الطبيعية في الاوساط الملحية |
| 264 #0 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Date of production, publication, distribution, manufacture, or copyright notice | 2025. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 109 pages : |
| Other physical details | illustrations ; |
| Dimensions | 25 cm. + |
| Accompanying material | CD. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Source | rda content |
| 337 ## - MEDIA TYPE | |
| Media type term | Unmediated |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | volume |
| Source | rdacarrier |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc)-Cairo University, 2025. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Bibliography: pages 93-109. |
| 520 #3 - SUMMARY, ETC. | |
| Summary, etc. | Microbiome-mediated strategies for future stressed-agriculture entail exploration of repertoires of halophyte microbiota. Culturomics strategies are advanced to improve culturability and extend diversity of microbiota of Salicornia europaea L. The plant broth-based-seawater-culture medium (PBSW) was advanced for in vitro domestication of microbiota of endo-rhizosphere/endo-phyllosphere of S. europaea. Populations (Colony Forming Units, CFUs) and biomass production (Optical Density, OD) were monitored throughout successive steps of in vitro cultivation/domestication in liquid batch cultures. Culture-dependent methods were applied to cultivate and identify (16S rRNA gene sequencing) representative isolates; and culture-independent analyses, denaturing gradient gel electrophoresis and real time PCR, (DGGE/qPCR) for community composition.<br/>PBSW supported higher CFUs counts; and related to 16S rRNA gene copy numbers (qPCR), increased (ca, 44-fold) culturability compared to NaCl-salted-standard culture medium. Successive in vitro domestication/batch cultures boosted bacterial growth, diminished differences among tested culture media and shortened doubling times (DT). PCR-DGGE showed divergence in culturable community composition primarily attributed to culture media. 16S rRNA gene sequencing of representative isolates indicated: a) greater diversity in endo-phyllospherethan endo-rhizosphere; b) abundant phyla were Pseudomonadota / Bacillota /Actinomycetota; c) dominance of Halomonas among 15 genera identified; d) Gracilibacillus, Metabacillus, Mixta, Salinicoccus, Zhihengliuella, Marinobacter, Marinimicrobium and Planomicrobium were first reported/cultivated for S. europaea. In vitro domestication resulted in dominance of genera of Pseudomonadota/Bacillota for endo-phyllosphere and Halomonas sp. of Pseudomonadota for endo-rhizosphere. PBSW created in situ similis milieu for cultivation of halophyte bacteria, and enabled in vitrodomestication for propagating microbiota, instead of laborious construction of consortia of single isolates, for future SynCom applications. |
| 520 #3 - SUMMARY, ETC. | |
| Summary, etc. | تم تطوير بيئة تعتمد على عصارة النبات ومياه البحر (PBSW) لاستئناس و عزل ميكروبات المناطق الداخلية للجذور والأوراق لنبات الساليكورنيا في المختبر. تمت مراقبة أعداد المستعمرات البكتيرية (CFUs) وإنتاج الكتلة الحيوية (الكثافة الضوئية، OD600)) خلال خطوات متتالية من الزراعة في مزارع بيئية سائلة. استخدمت الطرق المعتمدة على العزل كما استخدمت التحليلات غير المعتمدة على الزراعة (DGGE/qPCR) .دعمت بيئة PBSW أعدادًا أعلى من CFUs؛ وبالنسبة لنسخ جين 16S rRNA (qPCR)، زادت القابلية للعزل بحوالى 40 ضعفًا مقارنة بالبيئة الصناعية المدعمة بـملح NaCl . عززت تقنية الاستئناس المتتالية في المختبر نمو البكتيريا، وقلل الفروقات بين البيئات المختبرة، وقصّر وقت تضاعف خلايا الميكروبات.(DT) أظهرت تقنية PCR-DGGE تباينًا في تركيب المجتمع القابل للعزل حيث يُعزى الاختلاف أساسًا إلى نوع البيئات المستخدمة. أشارت نتائج 16S rRNA للعزلات الممثلة إلى:<br/>أ) تنوع أكبر في الميكروبات الداخلية للأوراقendo-phyllosphere مقارنة بالجذورendo-rhizosphere ب) الفصائل البكتيرية الأكثر وفرة هي Pseudomonadota وBacillota وActinomycetota ج) سيطرة جنس Halomonas بين 15 جنسًا محددًا؛<br/>د) تم االعزل لأول مرة عن أجناس Gracilibacillus وMetabacillus وMixta وSalinicoccus وZhihengliuella وMarinobacter وMarinimicrobium و Planomicrobium في نبات الساليكورنيا. |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Issues CD | Issues also as CD. |
| 546 ## - LANGUAGE NOTE | |
| Text Language | Text in English and abstract in Arabic & English. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Microbiology |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | الميكروبيولوجيا |
| 653 #0 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Halophyte microbiota |
| -- | Salicornia europaea |
| -- | Microbiota in vitro- domestication |
| -- | Culturomics of halophyte microbiota |
| -- | Mariout lake-Alexandria-Egypt halophytes |
| -- | Biomass production of halophyte microbiota |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nabil Abrahim Hegazi |
| Relator term | thesis advisor. |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohamed Fayez Fouad |
| Relator term | thesis advisor. |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tarek Sayed Ragab |
| Relator term | thesis advisor. |
| 900 ## - Thesis Information | |
| Grant date | 01-01-2025 |
| Supervisory body | Nabil Abrahim Hegazi |
| -- | Mohamed Fayez Fouad |
| -- | Tarek Sayed Ragab |
| Universities | Cairo University |
| Faculties | Faculty of Agricultural |
| Department | Department of Agricultural Microbiology |
| 905 ## - Cataloger and Reviser Names | |
| Cataloger Name | Shimaa |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Edition | 21 |
| Suppress in OPAC | No |
| Source of classification or shelving scheme | Home library | Current library | Date acquired | Inventory number | Full call number | Barcode | Date last seen | Effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 10.11.2025 | 92445 | Cai01.07.06.M.Sc.2025.Na.B | 01010110092445000 | 10.11.2025 | 10.11.2025 | Thesis |